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Protocols

Neo-Fusion specifications

Information


Unique identifier OMICS_32773
Name Neo-Fusion
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Programming languages C#
License MIT License
Computer skills Medium
Version 0.1.0
Stability Stable
Maintained Yes

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  • person_outline Lloyd Smith

Publication for Neo-Fusion

Neo-Fusion citations

 (35)
library_books

Differentially expressed microRNAs in diapausing versus HCl treated Bombyx embryos

2017
PLoS One
PMCID: 5507411
PMID: 28700597
DOI: 10.1371/journal.pone.0180085

[…] s and negative control (NC) mimics were purchased from Dingguo (Beijing, China). Dual luciferase expression vector pmirGlo (Promega, ) includes firefly luciferase (Luc2) and Renilla luciferase (hRluc-neo fusion). BmSDH 3’UTR (746 bp) was cloned and inserted into the Luc2 3’UTR to construct wild type (WT) plasmid, while Bmo-miR-2761-3p inhibitor was inserted into Luc2 3’UTR to construct positive co […]

library_books

A sestrin dependent Erk/Jnk/p38 MAPK activation complex inhibits immunity during ageing

2017
Nat Immunol
PMCID: 5321575
PMID: 28114291
DOI: 10.1038/ni.3665

[…] ined from EUCOMM. These cells were created by targeted gene trap approach and contain reporter-tagged insertion within intron 5 of the Sesn1 gene with a strong splice acceptor site expressing a β-Gal-Neo fusion protein that disrupts the Sesn1 ORF. The Sesn1+/- mice, produced from ES cells, were backcrossed again to wild-type C57BL/6 mice (n = 5) to eliminate any non-specific genetic aberrations th […]

library_books

Hydrogen peroxide induces La cytoplasmic shuttling and increases hepatitis C virus internal ribosome entry site dependent translation

2016
PMCID: 5042130
PMID: 27436793
DOI: 10.1099/jgv.0.000556

[…] CV sub-genomic replicon resembling a persistent infection () (). The sub-genomic replicon consists of a bicistronic transcript in which expression of the core (1–15 aa)-neomycin phosphotransferase (C-neo) fusion protein is under the translational control of the HCV IRES, whereas expression of the HCV NS3-NS5B is under the translational control of the encephalomyocarditis virus (EMCV) IRES. The eff […]

library_books

Gene Editing for the Efficient Correction of a Recurrent COL7A1 Mutation in Recessive Dystrophic Epidermolysis Bullosa Keratinocytes

2016
Mol Ther Nucleic Acids
PMCID: 5014520
PMID: 27045209
DOI: 10.1038/mtna.2016.19

[…] DEB-E67A6 keratinocytes transduced with Ad-TALENs vectors. Figure S4. AAV targeting vectors. Figure S5. PCR genotyping by amplification of selection cassette-genome junctions. Figure S6. COL7A1/2A-neo fusion transcript in recombinant clones obtained by transduction with the SA-2A-neo-AAV vector. Figure S7. Genotyping c.6527insC mutation correction. Figure S8. Neo selection cassette excision. […]

library_books

Prenatal ethanol exposure alters adult hippocampal VGLUT2 expression with concomitant changes in promoter DNA methylation, H3K4 trimethylation and miR 467b 5p levels

2015
PMCID: 4587775
PMID: 26421062
DOI: 10.1186/s13072-015-0032-6

[…] ng the pmirGLO vector system (Promega, USA) according to the manufacturer’s instructions. pmirGLO contains both a firefly luciferase reporter gene (luc2) and a Renilla luciferase reporter gene (hRluc-neo fusion). Inserts with PmeI (5′) and XbaI (3′) sticky ends were generated by annealing 2 μg of sense oligonucleotides and 2 μg of antisense oligonucleotides (Additional file : Table S3) in 46 μl of […]

library_books

The caveolin–cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle

2015
PMCID: 4555827
PMID: 26323694
DOI: 10.1083/jcb.201501046

[…] Cavin-1−/− mice, generated by replacing part of Exon 1 of the cavin-1 gene with a LacZ/Neo fusion cassette, were obtained from Boston University School of Medicine (). Cavin-1−/− mice, WT controls (both backcrossed onto the same C57BL/6 strain for 10 generations), and adult zebrafish we […]


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Neo-Fusion institution(s)
Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA
Neo-Fusion funding source(s)
Supported by the National Cancer Institute of the National Institutes of Health (NIH) under award numbers U24CA199347 and R01CA193481.

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