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NetCTL specifications


Unique identifier OMICS_05740
Name NetCTL
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Morten Nielsen

Publication for NetCTL

NetCTL citations


Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach

Sci Rep
PMCID: 5934409
PMID: 29725106
DOI: 10.1038/s41598-018-25374-z

[…] Cytotoxic T lymphocyte (CTL) epitopes prediction is essential for subunit vaccine development. NetCTL 1.2 was utilized to predict the CTL epitopes for hemolin protein based on MHC class-I binding affinity, TAP transport efficiency and proteasomal C-terminal cleavage. Scores of all three predict […]


Computational identification, characterization and validation of potential antigenic peptide vaccines from hrHPVs E6 proteins using immunoinformatics and computational systems biology approaches

PLoS One
PMCID: 5929558
PMID: 29715318
DOI: 10.1371/journal.pone.0196484

[…] Cytotoxic T-lymphocyte (CTL) epitopes were explored from the E6 proteins of the selected hrHPVs. NetCTL 1.2 server [] was utilized to predict the CTL epitopes. MHC binding affinity, proteasomal C-terminal cleavage, TAP transport affinity and potential MHC ligands were recognized by following > 0. […]


The perfect personalized cancer therapy: cancer vaccines against neoantigens

PMCID: 5910567
PMID: 29678194
DOI: 10.1186/s13046-018-0751-1

[…] ing work has been done in the past [–]. A few notable exceptions do exist but they start the analysis from preprocessed FASTA files, such as NetTepi ( and NetCTL (, including also a prediction method for T cell reactivity. It is reasonable to think that the integration of these additional modules would increa […]


Immunopharmacogenomics towards personalized cancer immunotherapy targeting neoantigens

Cancer Sci
PMCID: 5834780
PMID: 29288513
DOI: 10.1111/cas.13498
call_split See protocol

[…] dividual patients, and it has been reported that peptide–HLA complex stability might have a better correlation with the immunogenicity of the peptides. Some computational methods, such as NetChop and NetCTL, focus on predicting antigen processing and peptide transport processes., Structural‐based approaches are also important to predict the orientation of a mutated amino acid that is facing out o […]


Mapping HLA A2, A3 and B7 supertype restricted T cell epitopes in the ebolavirus proteome

BMC Genomics
PMCID: 5780746
PMID: 29363421
DOI: 10.1186/s12864-017-4328-8

[…] hers. Earlier studies had elucidated putative T-cell epitopes of individual EBOV proteins [–] by use of bioinformatics tools, such as SYFPEITHI [, ], BIMAS [, ], IEDB [, ], NetMHCcons [], NetChop [], NetCTL [], NetCTLpan [], NetMHCpan [] and NetMHCIIpan []. However, the identification and analyses of T-cell epitopes from the complete ZEBOV proteome, especially in the context of HLA-supertype restr […]


Novel Immunoinformatics Approaches to Design Multi epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Sci Rep
PMCID: 5773588
PMID: 29348555
DOI: 10.1038/s41598-018-19456-1
call_split See protocol

[…] inside the hepatocytes cells. To get an immunogenic CTL epitopes having the ability to elicit cell-mediated immunity and form the memory cells, all 14 salivary protein sequences were subjected to the NetCTL 1.2 server. NetCTL 1.2 is an online web server intended for predicting CTL epitopes among input protein sequences based on the training dataset. NetCTL was selected to predict the CTL epitopes […]


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NetCTL institution(s)
Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Lyngby, Denmark

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