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NetPhos specifications


Unique identifier OMICS_02434
Name NetPhos
Interface Web user interface
Restrictions to use None
Input data A single protein sequence or several protein sequences.
Input format FASTA
Computer skills Basic
Version 3.1
Stability Stable
Maintained Yes


  • person_outline Nikolaj Blom

Additional information

A version for kinase specific prediction of phosphorylation sites is also available at

Publications for NetPhos

NetPhos citations


Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis

Sci Rep
PMCID: 5954063
PMID: 29765103
DOI: 10.1038/s41598-018-26046-8

[…] and an online database for the prediction of Calmodulin binding sites ( The prediction of phosphorylation sites in EgPCDUF4201 was done by using NetPhos 2.0 server ( […]


c Src kinase is involved in the tyrosine phosphorylation and activity of SLC11A1 in differentiating macrophages

PLoS One
PMCID: 5933793
PMID: 29723216
DOI: 10.1371/journal.pone.0196230

[…] increased transcription and/or activity of proteins involved in cell growth and proliferation, ion transport, cell motility and invasion [–].Analysis of the amino acid sequence of human SLC11A1 using Netphos 2.0 server [] and GPS2.1 [] revealed the presence of two potential tyrosine phosphorylation sites (Y15 and Y38) at its N-terminus. In vitro phosphorylation assay have shown that murine Slc11a1 […]


Pir2/Rnf144b is a potential endometrial cancer biomarker that promotes cell proliferation

PMCID: 5938710
PMID: 29724995
DOI: 10.1038/s41419-018-0521-1

[…] ssess whether PIR2 expression is regulated downstream of a kinase signalling pathway, we first performed an in silico analysis to investigate the presence of potential phosphorylation sites. By using NetPhos 2.0 ( services/NetPhos/), we identified 3 potential serine phosphorylation sites (S165, S250 and S301) and 3 potential threonine phosphorylation sites (T44, T119 and T128 […]


Computational Protein Phenotype Characterization of IL10RA Mutations Causative to Early Onset Inflammatory Bowel Disease (IBD)

Front Genet
PMCID: 5934427
PMID: 29755507
DOI: 10.3389/fgene.2018.00146

[…] The NetPhos 2.0 server produces neural network predictions for phosphorylation sites in eukaryotic proteins. Neural networks can classify even non-linear and highly complex biological sequence patterns, w […]


Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinate mononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stages

Malar J
PMCID: 5896089
PMID: 29642910
DOI: 10.1186/s12936-018-2307-4

[…] The NetPhos 3.1 server [] predicted 21 possible phosphorylation sites for PfNMNAT: 15 for serine, 2 for tyrosine and 4 for threonine. To determine whether PfNMNAT is a phosphorylation target at some of it […]


BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice

Sci Adv
PMCID: 5938228
PMID: 29740613
DOI: 10.1126/sciadv.aar4471

[…] ion from the catalytic domain. In turn, Arf6 interacts with the catalytic domain of cytohesin-1. BIG1 has many potential phosphorylation sites involving tyrosine and serine/threonine kinases (see the NetPhos website, The activity of BIG1 may be regulated by phosphorylation.Hereditary peripheral neuropathies are associated with GEFs for Rho family GTPases. The mut […]


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NetPhos institution(s)
Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark; Department of Clinical Biochemistry, Glostrup Hospital, Glostrup, Denmark

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