NetPhos protocols

NetPhos specifications


Unique identifier OMICS_02434
Name NetPhos
Interface Web user interface
Restrictions to use None
Input data A single protein sequence or several protein sequences.
Input format FASTA
Computer skills Basic
Version 3.1
Stability Stable
Maintained Yes


  • person_outline Nikolaj Blom <>

Additional information

A version for kinase specific prediction of phosphorylation sites is also available at

Publications for NetPhos

NetPhos IN pipelines

PMCID: 5425475
PMID: 28553207
DOI: 10.3389/fnsys.2017.00028

[…] dm5-ht2b (figure 2). n-glycosylation sites were predicted by netnglyc 1.0 server1 and putative palmitoylation sites were predicted using gps-lipid2. putative phosphorylation sites were predicted by netphos 3.1 server3 (blom et al., 2004)., a comparison of the dm5-ht2b amino-acid sequence with ncbi databases identified several orthologous protostomian and deuterostomian 5-ht2 receptors. […]

PMCID: 5623054
PMID: 29018364
DOI: 10.3389/fphys.2017.00744

[…] the intracellular and n-glycosylation sites were predicted using neyglyc 1.0 server ( and the intracellular phosphorylation sites were predicted using netphos 3.1 server ( palmitoylation of cysteine residues were predicted by gps.lipid. an integrated resource for lipid modifications predictor […]

PMCID: 4066178
PMID: 24584662
DOI: 10.1007/s11033-014-3298-z

[…] domain and are passed via the secretory pathway without anchor sites, thus the two can be basically considered as secreted cas. other particular motifs are also present in the two sequences. the netphos 2.0 tool [24] identified 20 putative phosphorylation sites in f1 and 28 in f2. on the basis of the consensus sequences asn-xaa-ser/thr, the netnglyc 1.0 prediction tool revealed four […]

NetPhos institution(s)
Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark; Department of Clinical Biochemistry, Glostrup Hospital, Glostrup, Denmark

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