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NetPhosBac specifications


Unique identifier OMICS_17916
Name NetPhosBac
Interface Web user interface
Restrictions to use None
Input data A protein sequence.
Input format FASTA
Computer skills Basic
Version 1.0
Stability Stable
Maintained Yes


  • person_outline Ivan Mijakovic

Publication for NetPhosBac

NetPhosBac citations


Analysis of antibiotics resistant genes in different strains of Staphylococcus aureus

PMCID: 5953858
DOI: 10.6026/97320630014113

[…] SecretomeP was employed to perform non-classical and leaderless secretion of proteins. Serine and threonine phosphorylation sites in all the obtained antibiotic resistance genes were predicted using NetPhosBac 1.0 platform ( The Serine and threonine phosphorylation sites were validated using GPS 3.0 Mac version downloaded from ( […]


Molecular characterization of US like and Asian non S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013–2016 and PEDVs collected from recurrent outbreaks

PMCID: 5852955
PMID: 29540176
DOI: 10.1186/s12917-018-1409-0

[…] tes were predicted using a service available on Phosphorylation sites were predicted using the NetPhos 3.1 server ( and NetPhosBac 1.0 server ( Prediction of antigenic regions was performed using the EMBOSS protein analysis tool [] integrated into the Geneious software. […]


Virulence gene profiles: alpha hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

PMCID: 5834907
PMID: 29499697
DOI: 10.1186/s12917-018-1374-7

[…] es were aligned using BioEdit v7.0.9 (Micro Focus, Newbury, UK) to designate genotypes. Corresponding peptide sequences were deduced and aligned to determine the presence of amino acid substitutions. NetPhosBac 1.0 Server ( was used to predict serine and threonine phosphorylation sites in Hla peptide sequences, which were identified with non-synonymous […]


Multipronged regulatory functions of a novel endonuclease (TieA) from Helicobacter pylori

Nucleic Acids Res
PMCID: 5100599
PMID: 27550181
DOI: 10.1093/nar/gkw730

[…] Many translocated bacterial proteins undergo phosphorylation by host kinases at tyrosine, threonine and serine residues (). Therefore, we assessed whether TieA could also undergo phosphorylation; Netphosbac analysis ( illustrated that TieA protein sequence has the following serine residues: AECKSGELK, YINNSRR, RKNLSSLLY, KNLSSLLYE which could be p […]


US like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between 2013 and 2014

PMCID: 4668244
PMID: 26693114
DOI: 10.1186/s40064-015-1552-z

[…] three sites were all serine, one of few major amino acids that are capable of generating new O-linked glycosylation or phosphorylation. Netphos 2.0 server ( and NetPhosBac 1.0 Server ( were used for prediction of phosphorylation site. The result showed that phosphorylation was generated from serine residues at the […]


RD 1 encoded EspJ protein gets phosphorylated prior to affect the growth and intracellular survival of mycobacteria

Sci Rep
PMCID: 4521147
PMID: 26228622
DOI: 10.1038/srep12717

[…] lation potential as well as putative phosphorylation sites in RD1 encoded proteins were predicted by publicly available online analysis tool (); Netphos 2.0 (, NetPhosBac1 (, DISHPHOS 1.3 ( and, Kinasephos 2.0 ( Alignment of protei […]


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NetPhosBac institution(s)
Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark; Center for Microbial Biotechnology, Technical University of Denmark, Lyngby, Denmark; Department of Proteomics and Signal Transduction, Max-Planck-Institute for Biochemistry, Martinsried, Germany
NetPhosBac funding source(s)
This work was supported by grants from the Danish Natural Science Research Council (FNU) and Interaction Proteome, contract number: LSHG-CT-2003- 505520, European Commission FP6 Programme.

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