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NetPhosK specifications


Unique identifier OMICS_02435
Name NetPhosK
Interface Web user interface
Restrictions to use None
Computer skills Basic
Maintained Yes


  • person_outline Thomas Sicheritz Pontén <>

Publication for NetPhosK

NetPhosK citations


Identification and Functional Analysis of the First Aquaporin from Striped Stem Borer, Chilo suppressalis

PMCID: 5808226
PMID: 29467668
DOI: 10.3389/fphys.2018.00057

[…] bioinformatics, including translate, compute pi/mw, blast and tmhmm, were used to analyze the deduced csdrip1 protein sequence. phosphorylation and kinases sites were predicted using netphos 2.0 and netphosk 1.0, respectively (blom et al., , ). amino acid sequences were used to estimate phylogeny with the neighbor-joining, minimum evolution, maximum likelihood and maximum parsimony methods. […]


STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes

PMCID: 5738411
PMID: 29263348
DOI: 10.1038/s41598-017-18256-3

[…] using netphos 3.1, gps 3.0, or phosphosvm. under netphos 3.1, both generic- and kinase-specific predictions are performed, and the kinase-specific predictions by netphos 3.1 cover the prediction by netphosk 1.0. gps 3.0 predicts kinase-specific phosphorylations with a large-scale prediction of >13,000 mammalian phosphorylation sites and a proteome-wide prediction of aurora-b specific […]


Identification of a novel cAMP dependent protein kinase A phosphorylation site on the human cardiac calcium channel

PMCID: 5680263
PMID: 29123182
DOI: 10.1038/s41598-017-15087-0

[…] of the channel. comparative studies of available phosphorylation databases (kinexus phosphonet, phosphositeplus, phosida) and trials of in silico phosphorylation site prediction programs (netphosk 1.0, networkin, pkaps, gps 2.1, ppsp, scansite,) were used to predict pka substrate sites. considering direct enzyme-substrate interactions when a serine was preceded by an arginine at −3 […]


A Novel Phosphorylation Site Kinase Network Based Method for the Accurate Prediction of Kinase Substrate Relationships

PMCID: 5660750
PMID: 29312990
DOI: 10.1155/2017/1826496

[…] potential false positive to further improve performance. for example, linding et al. [] develop a web server, namely, networkin, which is based on known sequence motif extracted from scansite and netphosk, and the biological context of substrates is used as a filter to reduce false positives. meanwhile, to discover the potential protein kinases of the unannotated phosphorylation sites, song […]


Casein kinase 2 mediated phosphorylation of Brahma related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition

PMCID: 5682968
PMID: 28939766
DOI: 10.1074/jbc.M117.799676

[…] due to calcineurin inhibition (), suggesting that other phosphatases and kinases also regulate brg1 during myogenesis and almost certainly in other cell types. further analysis of brg1 sequence with netphosk predictor program () showed casein kinase 2 (ck2) as a putative kinase for brg1 (). ck2 is a ubiquitously expressed ser/thr kinase with over 300 identified substrates (, ). it is expressed […]


Petri Net Based Model of Helicobacter pylori Mediated Disruption of Tight Junction Proteins in Stomach Lining during Gastric Carcinoma

PMCID: 5592237
PMID: 28932213
DOI: 10.3389/fmicb.2017.01682

[…] (thr) and tyrosine (tyr) residues were predicted using netphos 2.0 (blom et al., ), based on artificial neural network programs, out of which exposed kinase specific sites were retrieved using netphosk (blom et al., ), kinasephos 2.0 (wong et al., ), and gps 2.1 (xue et al., ). sites verified by two or more databases were selected. obtained results were then scanned manually […]

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NetPhosK institution(s)
Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark

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