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Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana
[…] All primers for qPCR were designed based on D. montana genomic sequences using Primer3 (primer3.ut.ee) and NetPrimer (www.premierbiosoft.com/netprimer) programs (for primer sequences see Supplementary Table ). qPCR mix contained 10 µl of 2× Power SYBR Green PCR Master Mix (Bio-Rad Laboratories), 0.3 µM of […]
Multifunctional activities of ERF109 as affected by salt stress in Arabidopsis
[…] reactions using 1 ul cDNA, 1 × PCR buffer (with 1.5 mM MgCl2), 200 uM dNTPs, 200 nM of each gene-specific primers (Table ) and 0.2 U of Taq DNA polymerase (Promega Inc.). Primers were designed using Netprimer software (http://www.premierbiosoft.com/netprimer/index.html) with the following criteria: length 20–22 bases, GC content ~40–50%, minimal secondary structures, comparable annealing temperat […]
Ultrasound Activated Piezoelectric Nanoparticles Inhibit Proliferation of Breast Cancer Cells
[…] cDNA, were obtained with Primer. To prevent amplification from genome, we imposed that at least one primer spans an exon-exon boundary. Final validations for quality and specificity were performed on NetPrimer (PREMIER Biosoft International; http://www.premierbiosoft.com/netprimer/index.html), UCSC in silico PCR, and NCBI Blast. Primer sequences are shown in Table . […]
Genome analysis of the freshwater planktonic Vulcanococcus limneticus sp. nov. reveals horizontal transfer of nitrogenase operon and alternative pathways of nitrogen utilization
[…] enase genes (nifHDK) and of 16S rDNA of V.limneticus sp. nov. by real time PCR. The primers for all the tested genes were designed using the genome sequence of V.limneticus sp. nov. as template using NetPrimer software (http://www.premierbiosoft.com/netprimer/index.html), the specificity of each primer was verified by blasting all the primer sequences against that of the V.limneticus sp. nov. full […]
A comparison of the stem cell characteristics of murine tenocytes and tendon derived stem cells
[…] e Ct method [–]. Murine specific primers were used (Table ) and GAPDH was used as an internal control. Primers were designed using Primer-BLAST (NCBI), and the quality of each primer was tested using NetPrimer (Premier Biosoft). In addition, each primer was subjected to a BLAST (NCBI) search to ensure specificity. The best housekeeping gene was determined using the geNorm algorithm  and all prim […]
KELCH F BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME INTERACTING FACTOR1
[…] Primers for real-time qPCR were made to ACTIN2, PIF1, and CTG10 (Table S1) cDNA using IDT PrimerQuest (Integrated DNA Technologies, Inc.) and the Primer3 program (, ) and were analyzed using NetPrimer (PREMIER Biosoft; www.premierbiosoft.com/netprimer/). A Bio-Rad Laboratories iCycler permitted measurement of SYBR Green fluorescence to determine abundance of the PIF1 and CTG10 transcripts […]
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