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Citations per year

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NetStart specifications


Unique identifier OMICS_09991
Name NetStart
Interface Web user interface
Restrictions to use None
Input data Sequence(s)
Input format FASTA
Computer skills Basic
Version 1.0
Stability Stable
Maintained Yes


  • person_outline Anders Gorm Pedersen

Publication for NetStart

NetStart citations


A donor splice site mutation in CISD2 generates multiple truncated, non functional isoforms in Wolfram syndrome type 2 patients

BMC Med Genet
PMCID: 5729406
PMID: 29237418
DOI: 10.1186/s12881-017-0508-2

[…] exon 2 (Fig. , a2). Computer analysis performed on this small segment of genomic DNA (Chr4:102,861,131–102,861,212) did not identify the existence of any genes. In both variants, in silico analysis (NetStart1, predicted a translational start site in the beginning of exon 2 which resulted in a frame shift, introducing amino acids changes and a premature stop codon (Fig. , a1 […]


Transductive learning as an alternative to translation initiation site identification

BMC Bioinformatics
PMCID: 5290616
PMID: 28152994
DOI: 10.1186/s12859-017-1502-6

[…] ameters and the adopted validation process is presented. The “” section presents the results obtained by the comparative process between the ISVM and TSVM classifiers and a comparative study with the Netstart, TISHunter and TIS Miner programs. Finally, the “” section presents the final considerations. […]


Characterization of Alstrom Syndrome 1 (ALMS1) Transcript Variants in Hodgkin Lymphoma Cells

PLoS One
PMCID: 5279758
PMID: 28135309
DOI: 10.1371/journal.pone.0170694

[…] he first possible open reading frame will start in exon three. Analysis with translation start site prediction tools [,] indicated that the corresponding ATG codon in exon 3 can serve as start codon (NetStart 1.0 [] prediction scores for exon 1: 0.890, score for exon 3: 0.518; ATGpr [] reliability for exon 1: 0.60, for exon 3: 0.13). Alternatively, an additional exon upstream of exon one might exi […]


Illegitimate translation causes unexpected gene expression from on target out of frame alleles created by CRISPR Cas9

Sci Rep
PMCID: 5175197
PMID: 28000783
DOI: 10.1038/srep39608

[…] s a useful tool to investigate the detailed molecular mechanisms of ITL ().Our findings support the need for the careful design of genome-editing vectors for complete null mutations. For example, the NetStart 1.0 prediction server can be used to search for potential ITL initiation codons when designing vectors for genome editing. For Gli3, in addition to the native initiation codon at +1, there ar […]


Biosynthesis of Antibiotic Leucinostatins in Bio control Fungus Purpureocillium lilacinum and Their Inhibition on Phytophthora Revealed by Genome Mining

PLoS Pathog
PMCID: 4946873
PMID: 27416025
DOI: 10.1371/journal.ppat.1005685
call_split See protocol

[…] 1 genes of the PLBJ-1 and PLFJ-1 strains were obtained according to the method in []. EuGene, version 4.1 [], was used to integrate multiple sources, including transcription start sites identified by Netstart [], homologous proteins identified from the Swiss-Prot database, version 2015-07-22, by BLAST, version 2.2.26, the assembled transcripts generated by IDBA-tran, version 1.1.1 [], and the exon […]


LncRNA HIT Functions as an Epigenetic Regulator of Chondrogenesis through Its Recruitment of p100/CBP Complexes

PLoS Genet
PMCID: 4669167
PMID: 26633036
DOI: 10.1371/journal.pgen.1005680

[…] odons present in the LncRNA-HIT transcript also suggested poor protein coding potential, as these sites lack a Kozak consensus sequence and exhibited poor translational potential as determined by the NetStart software package (),, [–].Localization of the LncRNA-HIT transcript by in situ hybridization revealed expression in the pre-chondrogenic limb mesenchy […]

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NetStart institution(s)
Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark

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