Neurolucida protocols

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chevron_left Morphometric analysis Cell counting Neuron tracing Neurite growth Neuron tracing Morphometric analysis Cell counting Neuron tracing Cell counting Image acquisition Neurite growth chevron_right
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Neurolucida specifications

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Unique identifier OMICS_06592
Name Neurolucida
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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  • person_outline Neurolucida Team <>

Neurolucida in pipelines

 (3)
2012
PMCID: 3283560
PMID: 22363206
DOI: 10.1371/journal.pbio.1001262

[…] processing software imaris 5.0 (bitplane ag, zurich, switzerland) was used to measure the spine head volume and neck length (see also ) . for spine density analysis, dendrites were traced with the neurolucida 7.1 computerized data collection system. spine density was automatically calculated by dividing the number of spines on a dendrite by the dendrite length. see for further details., […]

2011
PMCID: 3558750
PMID: 22179109
DOI: 10.1038/nn.3004

[…] using a lsm510 meta confocal microscope and a 63× oil-immersion objective (zeiss, na: 1.4). the basal and apical dendrites of well-stained neurons were reconstructed in three dimensions using a neurolucida system (microbrightfield, williston, vt). no correction was made for tissue shrinkage., all drugs were obtained from tocris (ellisville, mo, usa) and used in the following concentrations […]

2009
PMCID: 2682575
PMID: 19479070
DOI: 10.1371/journal.pone.0005655

[…] perform or monitor the tracing. such programs include the neuronj plug-in to the open-source imagej platform , as well as commercial packages such as imaris (bitplane ag, zurich, switzerland), neurolucida (microbrightfield, inc., williston, vt), amira (mercury computer systems, inc., chelmsford, ma), and volocity (improvision inc., lexington, ma)., these software packages do not perform […]


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Neurolucida in publications

 (876)
PMCID: 5955882
PMID: 29769664
DOI: 10.1038/s41598-018-25740-x

[…] were fixed, sectioned and processed with avidin-biotin-peroxidase complex (abc) for bda visualization. dendrites and axons of mtcs were three-dimensionally reconstructed from digital data using neurolucida., whole-cell current-clamp recordings were made from mtcs in acute horizontal slices (310 μm thick) prepared from ~4 w-old c57bl/6 mice of both sexes as previously described. action […]

PMCID: 5943589
PMID: 29743575
DOI: 10.1038/s41598-018-25475-9

[…] injured cortex were stained with cresyl violet to show the spare cortex. the boundary contours of the contralateral and ipsilateral spare cortex were drawn with a zeiss microscope attached to a neurolucida system (microbrightfield inc., colchester, vt). the enclosed volume within the contours was measured. the percent cortex of the cavity was calculated with the following […]

PMCID: 5940682
PMID: 29740022
DOI: 10.1038/s41598-018-25354-3

[…] 2 brains from wt (n = 10 from 5 brains) and arhgap15−/− (n = 10 from 5 brains). after 7 days of diffusion time, we examined the dendritic complexity of dii-labelled callosal neurons (fig. ) using the neurolucida software (fig. ). in the arhgap15−/− brains we detected a reduced length of axons (wt 0.22 mm ± 0.03 vs. ko 0.11 mm ± 0.01) and dendrites (wt 0.22 mm ± 0.03 vs. ko 0.08 mm ± 0.02) (fig. ). […]

PMCID: 5935963
PMID: 29729668
DOI: 10.1186/s12974-018-1172-y

[…] diameter) using nucleator probe of stereo investigator system, whereas processes were reconstructed and analyzed in three dimensions within a single section using a computer-based tracing system (neurolucida, mbf bioscience, williston, vt). for tracing and reconstruction, 10–15 random cells per section were analyzed. similarly, stereological quantification of dcx-positive cells was counted […]

PMCID: 5945818
PMID: 29780306
DOI: 10.3389/fncel.2018.00120

[…] in ptw (1 h each), and fixed in 4% pfa for 30 min. samples were then cleared in 60% glycerol overnight and mounted on slides in 100% glycerol (fisher, hampton, nh, united states) and imaged with a neurolucida system (microbrightfield, inc., united states) integrated with a zeiss axioimager m2 microscope (carl zeiss microscopy, germany) or an olympus ix70 microscope (olympus corporation, […]


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