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Neurolucida specifications


Unique identifier OMICS_06592
Name Neurolucida
Alternative name Neurolucida 360
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes


  • Neurolucida Explorer


No version available


  • person_outline Neurolucida Team

Neurolucida citations


Hyperactivity of Rac1 GTPase pathway impairs neuritogenesis of cortical neurons by altering actin dynamics

Sci Rep
PMCID: 5940682
PMID: 29740022
DOI: 10.1038/s41598-018-25354-3
call_split See protocol

[…] vidual neurons stained with the DiI retrograde tracing method (see below). For these analyses, at least 50 neurons belonging to the same cortical area and layer were traced for each genotype with the Neurolucida software package. Neurons were analyzed using the Neurolucida Explorer software package (MBF Bioscience) quantifying the length of axon, dendrites and apical dendrite, and making the Sholl […]


URMC 099 facilitates amyloid β clearance in a murine model of Alzheimer’s disease

PMCID: 5935963
PMID: 29729668
DOI: 10.1186/s12974-018-1172-y
call_split See protocol

[…] nal diameter) using nucleator probe of Stereo Investigator system, whereas processes were reconstructed and analyzed in three dimensions within a single section using a computer-based tracing system (Neurolucida, MBF Bioscience, Williston, VT). For tracing and reconstruction, 10–15 random cells per section were analyzed. Similarly, stereological quantification of Dcx-positive cells was counted in […]


Diverse Long Range Axonal Projections of Excitatory Layer 2/3 Neurons in Mouse Barrel Cortex

Front Neuroanat
PMCID: 5938399
PMID: 29765308
DOI: 10.3389/fnana.2018.00033
call_split See protocol

[…] sing the sections in PBS. Finally, the slices were mounted on gelatinised Superfrost slides using Mowiol. Axonal and dendritic processes were subsequently reconstructed from the serial sections using Neurolucida software (MBF Bioscience).The DAB-stained neurons were reconstructed using an Olympus BX51WI microscope using an oil 60x lens (Olympus PlanApo 60x Oil NA 1.42) along with Neurolucida 64 bi […]


Src Kinase Dependent Rapid Non genomic Modulation of Hippocampal Spinogenesis Induced by Androgen and Estrogen

Front Neurosci
PMCID: 5938344
PMID: 29765299
DOI: 10.3389/fnins.2018.00282

[…] so-3D (automated software calculating mathematically geometrical parameters of spines) developed by Bioinformatics Project of Kawato's group (Mukai et al., ). Spiso-3D has an equivalent capacity with Neurolucida (MicroBrightField, USA), furthermore, Spiso-3D considerably reduces human errors and experimenter labor. The single apical dendrite was analyzed separately. The spine density was calculate […]


Automated sorting of neuronal trees in fluorescent images of neuronal networks using NeuroTreeTracer

Sci Rep
PMCID: 5915526
PMID: 29691458
DOI: 10.1038/s41598-018-24753-w

[…] ns, we recall that existing automated or semi-automated methods include several academic routines– and other freeware (e.g., Neuromantic, Neuronstudio,) or commercial software packages (e.g., Imaris, Neurolucida, PerkinElmer Cellular Imaging) offering multiple capabilities. Many current methods still require significant manual intervention, and their performance is typically very sensitive on the […]


MHC class I in dopaminergic neurons suppresses relapse to reward seeking

Sci Adv
PMCID: 5851664
PMID: 29546241
DOI: 10.1126/sciadv.aap7388

[…] sequential z-series sections. In each slice, two to three secondary dendrites (80 μm in length on average) were analyzed. Spine density, spine head diameter, and spine neck length were analyzed using Neurolucida (MBF Bioscience). There was no effect of genotype or treatment (cocaine/vehicle self-administration) on neck length (fig. S5F). […]


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Neurolucida institution(s)
MBF Bioscience, Williston, VT, USA; MBF Europe B.V., Delft, The Netherlands; MicroBrightField China, Kln, Hong Kong; MicroBrightField Japan, Chiba, Japan

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