The study of the structure and function of neuronal cells and networks is of crucial importance in the endeavor to understand how the brain works. A key component in this process is the extraction of neuronal morphology from microscopic imaging data.
A tool for creating and analyzing realistic, meaningful, and quantifiable neuron reconstructions from microscope images. Perform detailed morphometric analysis of neurons, such as quantifying: 1) the number of dendrites, axons, nodes, synapses, and spines, 2) the length, width, and volume of dendrites and axons, 3) the area and volume of the soma and 4) the complexity and extension of neurons.
Traces and analyzes neurites in fluorescence microscopy images. NeuronJ consists in a semi-automatic neurite tracing technique that employs a global optimization algorithm and second-order image feature analysis, making it robust against noise, varying or discontinuous background intensities, and varying or locally diminishing neurite contrast. It can thus be applied to a wide range of images without changing its parameters.
An application for semi-automated tracing of neurons to quickly annotate noisy datasets and construct complex neuronal topologies. Simple Neurite Tracer is designed to allow easy semi-automatic tracing of neurons or other tube-like structures (e.g. blood vessels) through 3D image stacks. The plugin has built-in tools for analysis and hardware accelerated 3D visualization of the results. Data can be imported and exported in SWC files for interaction with other software, or details of the traces can be exported as CSV files for analysis in spreadsheets or statistical software. The native file format is open and XML-based.
Assists in automating and multithreading Sholl for direct analysis of fluorescent images and traced morphologies. Sholl analysis is a plugin for the image analysis software ImageJ. It creates a serie of concentric circles around the soma of the neuron, and counts how many times the neuron intersects with the circumference of these circles. This software can be applied universally to gray-scale images of neurons of different shapes or sizes.
Allows users to visualize 3D/4D/5D image and to analyze system for bioimages and surface objects. Vaa3D consists of a container of modules for 3D image analysis (cell segmentation, neuron tracing, brain registration, annotation, quantitative measurement and statistics) and data management. Moreover, this platform can be used for developing 3D image analysis algorithms for high-throughput processing.
Handles tera-scale serial section electron microscopes (EM) image datasets for neural circuit rebuilding. TrakEM2 is a plugin that can be run through the ImageJ software. It includes multiple features allowing users to measure, visualize and annotate neuronal components. The application can be used for recording images from focused ion beam-scanning electron microscope (FIBSEM) or for reconstructing neuronal lineages and organs.
Allows reconstruction of neuronal structures from confocal and multi-photon images. NeuronStudio provides tools for manual, semi-manual, and automatic tracing of the dendritic arbor as well as manual and automatic detection and classification of dendritic spines. In addition, advanced 2D and 3D visualization techniques facilitate the verification of the reconstruction, as well as allowing accurate manual editing.