NeurphologyJ specifications


Unique identifier OMICS_06595
Name NeurphologyJ
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Stability Stable
Maintained Yes


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  • person_outline Eric Hwang <>

Publication for NeurphologyJ

NeurphologyJ in publications

PMCID: 5897719
PMID: 29361543
DOI: 10.1242/jcs.210393

[…] on the deltavision elite microscope (ge healthcare life sciences) using a 20× air objective (pln 20×/0.4, olympus). the analysis was performed by running the morphology quantification software neurphologyj, an imagej plugin, as described by ., colocalization analysis was performed by using the coloc2 image plugin, by following the described procedure ()., the viability plot was calculated […]

PMCID: 5390135
PMID: 28344003
DOI: 10.1016/j.stemcr.2017.02.020

[…] imagequant las 4000 mini system (ge healthcare)., neuron morphology, including soma size, neurite length, and neurite branching complexity, was automatically quantified using imagej (nih) with the neurphologyj plug-in ()., in all experiments, all available ipsc lines (three from the patient and three lines from two different control donors) were included. data from continuous variables […]

PMCID: 5304320
PMID: 28205572
DOI: 10.1038/srep42297

[…] milliseconds over a 2-minute period. only the neurons with clear eb3 comets were imaged., for neurite length analysis of p19 neurons, β-iii-tubulin stained images were analyzed using imagej plug-in neurphologyj. for neurite length analysis of hippocampal neurons, neurites were manually traced with the imagej plugin neuronj 1.4.1. only neurons expressing both egfp and β-iii-tubulin […]

PMCID: 4220284
PMID: 25370150
DOI: 10.1038/srep06919

[…] the culture surface. this is a reliable indicator of viable neurons, because toxin-induced cell death caused neurons to detach from the culture surface. the neuron morphology quantification software neurphologyj was used to quantify the number of neurons in hippocampal and drg neuron cultures after fnd treatment. we did not detect any significant change in hippocampal or drg neuron number […]

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NeurphologyJ institution(s)
Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan

NeurphologyJ review

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Anonymous user #1225's avatar image No country

Anonymous user #1225

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Dear All,

currently I am applying NeurphologyJ plugin (on ImageJ) in order to measure the projection length in neurons.
I follow the given instructions but in the last step of "Operation Instruction: Neurphology interactive application" I get the follow message "The source stacks must have the same number of images". Also I cannot see the "NeurphologyJHT in options at menu. Thus I cannot complete the analysis.
I am probably missing something or doing wrong.
do you have any ideas what I could do to fix it?

Thank you very much in advance,