NMRPipe protocols

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NMRPipe specifications


Unique identifier OMICS_02844
Name NMRPipe
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


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  • person_outline Frank Delaglio <>

Publication for NMRPipe

NMRPipe in pipelines

PMCID: 5539425
PMID: 28765220
DOI: 10.1128/mBio.00887-17

[…] spectrometer equipped with a quadrapole-resonance qci-p cryoprobe (1h, 13c, 15n, and 31p), a samplejet automated sample changer, bruker icon-nmr, and an automatic tuning and matching (atm) unit. nmrpipe () and nmrviewj () were used to process and analyze the collected spectra. the tmsp internal standard was used for chemical shift referencing and normalization of nmr peak intensities. nmr […]

PMCID: 4165433
PMID: 25228990
DOI: 10.1186/2049-3002-2-18

[…] points and a spectrum width of 4,735 hz in the direct dimension and 64 data points and a spectrum width of 17,607 hz in the indirect dimension. the 2d 1h-13c hsqc nmr spectra were processed using nmrpipe (nih, bethesda, maryland) [] and analyzed using nmrviewj version 8.0.3. peak intensities were normalized by the average peak intensity for a given spectrum and then assigned to a metabolite […]

NMRPipe in publications

PMCID: 5940772
PMID: 29739942
DOI: 10.1038/s41467-018-04214-8

[…] to a proton larmor frequency of 800 mhz. samples were measured at concentrations ranging between 0.1 and 0.5 mm in 20 mm na2hpo4, 150 mm nacl, 0.5 mm tcep at ph 6.5. all spectra were processed with nmrpipe and analysed using sparky, ccpnmr and cara (http://cara.nmr.ch). for backbone resonance assignments of the pryspry domain, hncacb, cbca(co)nh and hnca spectra were recorded. backbone […]

PMCID: 5902452
PMID: 29662055
DOI: 10.1038/s41467-018-03746-3

[…] 52 hz, respectively, were collected 256 t1 increments with 96 transients of 4096 points for each free induction decay. the experiment was acquired in an edited mode. all nmr data were processed using nmrpipe and analyzed with nmr view., the structure calculations were performed with the xplor-nih version 2.28 software by simulated annealing (sa) algorithm. noe intensities were converted into semi- […]

PMCID: 5890618
PMID: 29526435
DOI: 10.1016/j.str.2018.02.007

[…] perturbation assays were carried out at 298k using a bruker avance spectrometer operating at 950 mhz with a txi cryoprobe controlled by topspin 3 (bruker biospin ltd). spectra were processed using nmrpipe/nmrdraw () and analyzed using ccpnmr analysis 2.2 (). 100 μm 15n-labelled csfba48e in 50 mm hepes ph 7.5, 150 mm kcl, 0.5 mm tcep was titrated with unlabelled σg or σe up to a ratio of 1:2 […]

PMCID: 5883053
PMID: 29615624
DOI: 10.1038/s41467-018-03562-9

[…]  mm cypa and 10.5 mm suc-afpf-pna. sample temperatures were calibrated using the 4% methanol + 96% methanol-d4 sample (dlm-5007, cambridge isotope laboratories). all data sets were processed with the nmrpipe/nmrdraw software package and visualized/analyzed using the program nmrfam-sparky., trosy-versions of a 3d hncacb and cbca(co)nh experiments were recorded at 25 °c to obtain a nearly complete s […]

PMCID: 5908246
PMID: 29562181
DOI: 10.1016/j.celrep.2018.02.097

[…] a 5% fdr was used for the results from both searches. spectra were manually evaluated using the comet/lorikeet spectrum viewer (trans-proteomic pipeline) ()., all nmr spectra were processed using nmrpipe () and analyzed in nmrviewj (). nmr peak intensities for spin-label experiments were quantified using nmrviewj, and error bars reflect noise levels of the spectra. peak centers for rdc […]

NMRPipe institution(s)
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA

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