NMRPipe

[…] to a proton larmor frequency of 800 mhz. samples were measured at concentrations ranging between 0.1 and 0.5 mm in 20 mm na2hpo4, 150 mm nacl, 0.5 mm tcep at ph 6.5. all spectra were processed with nmrpipe and analysed using sparky, ccpnmr and cara (http://cara.nmr.ch). for backbone resonance assignments of the pryspry domain, hncacb, cbca(co)nh and hnca spectra were recorded. backbone […]

[…] 52 hz, respectively, were collected 256 t1 increments with 96 transients of 4096 points for each free induction decay. the experiment was acquired in an edited mode. all nmr data were processed using nmrpipe and analyzed with nmr view., the structure calculations were performed with the xplor-nih version 2.28 software by simulated annealing (sa) algorithm. noe intensities were converted into semi- […]

[…]  mm cypa and 10.5 mm suc-afpf-pna. sample temperatures were calibrated using the 4% methanol + 96% methanol-d4 sample (dlm-5007, cambridge isotope laboratories). all data sets were processed with the nmrpipe/nmrdraw software package and visualized/analyzed using the program nmrfam-sparky., trosy-versions of a 3d hncacb and cbca(co)nh experiments were recorded at 25 °c to obtain a nearly complete s […]

[…] perturbation assays were carried out at 298k using a bruker avance spectrometer operating at 950 mhz with a txi cryoprobe controlled by topspin 3 (bruker biospin ltd). spectra were processed using nmrpipe/nmrdraw () and analyzed using ccpnmr analysis 2.2 (). 100 μm 15n-labelled csfba48e in 50 mm hepes ph 7.5, 150 mm kcl, 0.5 mm tcep was titrated with unlabelled σg or σe up to a ratio of 1:2 […]

[…] a 5% fdr was used for the results from both searches. spectra were manually evaluated using the comet/lorikeet spectrum viewer (trans-proteomic pipeline) ()., all nmr spectra were processed using nmrpipe () and analyzed in nmrviewj (). nmr peak intensities for spin-label experiments were quantified using nmrviewj, and error bars reflect noise levels of the spectra. peak centers for rdc […]

[…] 0.13 mhz 1h, 150.90 mhz 13c, and 60.81 mhz 15n frequencies, equipped with a cryogenically cooled probe head. all data were collected at 298.0 k. the nmr 1d data sets were processed and analyzed using nmrpipe and nmrdraw, respectively., kinetic measurements of fab binding to the sosip.664 constructs were carried out using the octet qke system (fortebio); 0.2 mg/ml of each his-tagged fab was immobil […]