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NormaCurve specifications

Information


Unique identifier OMICS_00814
Name NormaCurve
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Version 1.0.0
Stability Stable
Maintained Yes

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  • person_outline Leanne de Koning <>

Publication for NormaCurve

NormaCurve in publications

 (5)
PMCID: 5534104
PMID: 28754127
DOI: 10.1186/s13014-017-0858-0

[…] microarray scanner (molecular devices, sunnyvale, ca, usa). spot intensity was determined with microvigene software (vigenetech inc., carlisle, ma, usa). quantification of the data was achieved with normacurve [], which includes data normalization against negative control slides and sypro ruby slides., statistical analysis was performed using r v2.15.1 (http://www.cran.r-project.org). the tests […]

PMCID: 4984455
PMID: 27172249
DOI: 10.1038/bjc.2016.123

[…] inc., carlisle, ma, usa). all primary antibodies used in rppa have been previously tested by western blotting to assess their specificity for the protein of interest., raw data were normalised using normacurve (), which normalises for fluorescent background per spot, a total protein stain and potential spatial bias on the slide. next, each rppa slide was median centred and scaled (divided […]

PMCID: 4700448
PMID: 26729235
DOI: 10.1038/srep18517

[…] arrays were labelled as described in rondeau et al.. specificity of each primary antibody was first validated by western blotting on a panel of breast cancer cell lines. data were normalized using normacurve software. bias due to origin of the samples (nki vs cam) was removed using a median regression approach., we profiled a panel of 15 ilc-like cell lines (). drug sensitivity was assessed […]

PMCID: 4587544
PMID: 25822088
DOI: 10.1038/ng.3252

[…] replicates per dilution were deposited for each sample. arrays were revealed with an anti-nqo1 antibody (hpa007308 from sigma). quantification and normalization of rppa data was performed using the normacurve method., nqo1 p187s (nqo1*2 allele, rs1800566) snp genotyping was performed on genomic dna extracted from cells using a pre-designed taqman assay (c_2091255_30, life technologies), […]

PMCID: 4366900
PMID: 25742469
DOI: 10.1038/bjc.2015.60

[…] inc., carlisle, ma, usa). all primary antibodies used in rppa have been previously tested by western blotting to assess their specificity for the protein of interest., raw data were normalised using normacurve (), which normalises for fluorescent background per spot, a total protein stain and potential spatial bias on the slide. next, each rppa slide was median centred and scaled (divided […]


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NormaCurve institution(s)
Institut Curie, Paris, France; Department of Translational Research, Institut Curie, Paris, France; INSERM, U900, Paris, France; Mines ParisTech, Fontainebleau, France; CNRS UMR144, Paris, France

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