bookmark flowBin A package based on a parameterization-free method for combining multitube FCM data into a higher-dimensional form suitable for deep profiling and discovery. FlowBin allocates cells to bins defined by the common markers across tubes in a multitube experiment, then computes aggregate expression for each bin within each tube, to create a matrix of expression of all markers assayed in each tube. It is designed to accept multiple FCM assays from the same multitube assay and combine these into a complete matrix of measurements for all the markers. To this end, flowBin consists of four stages: (i) normalization, (ii) binning, (iii) bin matching across tubes and (iv) expression measurement. Compared with NNs merging of tubes, flowBin produces cleaner data, with far fewer false double-positive marker combinations.