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NormFinder specifications


Unique identifier OMICS_02317
Name NormFinder
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


No version available

Publication for NormFinder

NormFinder citations


Transcriptome Analysis of Circulating PBMCs to Understand Mechanism of High Altitude Adaptation in Native Cattle of Ladakh Region

Sci Rep
PMCID: 5955995
PMID: 29769561
DOI: 10.1038/s41598-018-25736-7
call_split See protocol

[…] qPCR data of target genes in high altitude and tropically adapted cattle was normalized by selecting best suited ICGs utilizing geNorm, NormFinder and Best keeper softwares–.The mean value was calculated using relative quantification 2−ΔΔCT method. The differences between groups were tested by Tukey’s Multiple Comparison Test. P value […]


Pheromone gland development and monoterpenoid synthesis specific to oviparous females in the pea aphid

PMCID: 5946545
PMID: 29780614
DOI: 10.1186/s40851-018-0092-0

[…] lyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, elongation factor 1 alpha (EF1a), and ribosomal protein L32 (rpL32), by three statistical software programs, geNorm [], BestKeeper [], and Normfinder []. As the results, the expression level of GAPDH was shown to be the most stable so that it was used as the reference gene.Data acquisition and analyses were performed by ABI Prism 7500 v2 […]


An overview of technical considerations when using quantitative real time PCR analysis of gene expression in human exercise research

PLoS One
PMCID: 5944930
PMID: 29746477
DOI: 10.1371/journal.pone.0196438
call_split See protocol

[…] a statistical analysis on the Cq value or using available software. Several software programs are available for reference gene evaluation using different analytical approaches, such as BestKeeper [], NormFinder [], and GeNorm [].In our laboratory, we have six commonly-used reference genes, ACTB, TATA-box binding protein (TBP), Cyclophilin, GAPDH, B2M, and 18S rRNA (). There are a few reasons why t […]


Selection and Validation of Reference Genes for Quantitative Real Time PCR Normalization Under Ethanol Stress Conditions in Oenococcus oeni SD 2a

Front Microbiol
PMCID: 5946679
PMID: 29780378
DOI: 10.3389/fmicb.2018.00892

[…] NormFinder analysis result was slightly different from that generated by geNorm (Table ). According to NormFinder analysis, dnaG and gyrA were ranked in top positions across all samples, but ranked se […]


Selection and evaluation of housekeeping genes as endogenous controls for quantification of mRNA transcripts in Theileria parva using quantitative real time polymerase chain reaction (qPCR)

PLoS One
PMCID: 5935388
PMID: 29727459
DOI: 10.1371/journal.pone.0196715

[…] ability of the HKGs and rank them accordingly ( ReFfinder evaluates the reference gene stability through integrated computational programs, including geNorm, Normfinder, BestKeeper. The program then uses the comparative delta Ct method to compare and rank specific candidate endogenous control genes. Subsequently it then assigns an appropriate weight to eac […]


The dehydration stress of couch grass is associated with its lipid metabolism, the induction of transporters and the re programming of development coordinated by ABA

BMC Genomics
PMCID: 5930771
PMID: 29720087
DOI: 10.1186/s12864-018-4700-3

[…] d the geometric mean is calculated based on a particular weighting. The genes with the lowest value are considered the most stable. The final ranking of candidate reference genes according to GeNorm, NormFinder and BestKeeper as well as RefFinder is displayed in Additional file : Table S2.To find out the optimal number of reference genes, we applied the pairwise variation (V) within GeNorm (see Ad […]


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NormFinder institution(s)
Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, Aarhus, Denmark

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