NormqPCR statistics

info info

Citations per year

info

Popular tool citations

chevron_left Data analysis Reference genes chevron_right
info

Tool usage distribution map

Tool usage distribution map
info info

Associated diseases

Associated diseases
Want to access the full stats & trends on this tool?

NormqPCR specifications

Information


Unique identifier OMICS_02315
Name NormqPCR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
License GNU Lesser General Public License version 3.0
Computer skills Advanced
Version 1.26.0
Stability Stable
Requirements
methods, stats, RColorBrewer, Biobase, R(>=2.14.0), qpcR, ReadqPCR
Maintained Yes

Versioning


No version available

Documentation


Publication for NormqPCR

NormqPCR citations

 (27)
library_books

Selection and validation of reference genes for quantitative gene expression analyses in black locust (Robinia pseudoacacia L.) using real time quantitative PCR

2018
PLoS One
PMCID: 5846725
PMID: 29529054
DOI: 10.1371/journal.pone.0193076

[…] [], poplar [–],eucalyptus [,], and teak []. to determine which reference gene is best for transcript normalization in a given subset of biological samples, statistical algorithms such as reffinder, normqpcr, refgenes, oliver, genorm, bestkeeper, and normfinder have been developed (https://omictools.com/reference-genes-category) [,]., we used statistical and graphical methods to compare […]

library_books

Timing of embryonic quiescence determines viability of embryos from the calanoid copepod, Acartia tonsa (Dana)

2018
PLoS One
PMCID: 5841787
PMID: 29513715
DOI: 10.1371/journal.pone.0193727

[…] different genes to be the most stable across different developmental stages (eggs, adults) and stressors (salinity shock, handling—and density stress) after assessment with the genorm method in the normqpcr r package []., to ensure the identity of the primer product sequences originated from the a. tonsa transcriptome, conventional pcr were used to generate pcr products and submitted […]

library_books

High Expression of TET1 Predicts Poor Survival in Cytogenetically Normal Acute Myeloid Leukemia From Two Cohorts

2018
PMCID: 5835576
PMID: 29402726
DOI: 10.1016/j.ebiom.2018.01.031

[…] the commonly used housekeeping (hk) genes like β-actin, α-tubulin, gusb and gapdh were measured. in order to select the suitable hk genes in our cohort, we performed genorm analysis using r package “normqpcr” (). as a result, the m values of β-actin, α-tubulin, gusb and gapdh were 0.11, 0.08, 0.08 and 0.07, respectively. thus, gapdh was selected as the most stable hk gene owing to the lowest […]

library_books

Small ocean temperature increases elicit stage dependent changes in DNA methylation and gene expression in a fish, the European sea bass

2017
Sci Rep
PMCID: 5622125
PMID: 28963513
DOI: 10.1038/s41598-017-10861-6

[…] 1.2.1 (applied biosystems). measured cq values were corrected for primer efficiency cqe = cq(log(1+e)/log2) and further analyses were performed using the r packages readqpcr (version 1.16.0) and normqpcr (version 1.16.0). the geometric mean of the two reference genes was used to normalize the expression of target genes. relative gene expression was calculated using the 2−δδcq method […]

library_books

Two microRNA signatures for malignancy and immune infiltration predict overall survival in advanced epithelial ovarian cancer

2017
PMCID: 5847100
PMID: 28716985
DOI: 10.1136/jim-2017-000457

[…] micrornas for analysis. raw ct expression values were normalized using the ∆ct method, with the top 10 housekeeping micrornas obtained from the genorm algorithm, implemented in the r bioconductor normqpcr package, v.1.20 mrna profiling. mrna profiles were generated using illumina’s whole genome gene expression dasl ht assay. approximately 200 ng of rna from 19 primary ovarian cancer tissue […]

library_books

Periovulatory follicular fluid levels of estradiol trigger inflammatory and DNA damage responses in oviduct epithelial cells

2017
PLoS One
PMCID: 5322925
PMID: 28231273
DOI: 10.1371/journal.pone.0172192

[…] gene expression was calculated by applying the 2-δδct method [], corrected for pcr efficiency. reference genes for normalization were determined using the genorm algorithm, part of the r package “normqpcr” []., data from poec was tested for normality (shapiro wilk test). normal data was analyzed by repeated-measures analysis of variance (anova), followed by pairwise paired t-test […]


Want to access the full list of citations?
NormqPCR institution(s)
Institute of Structural and Molecular Biology, University College of London, London, UK

NormqPCR review

star_border star_border star_border star_border star_border
star star star star star

jiang tao

star_border star_border star_border star_border star_border
star star star star star
Desktop
I wang to install the ReadqPCR, "package ‘ReadqPCR’ is not available (for R version 3.4.3)". So disappointed.