NormqPCR statistics

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NormqPCR specifications

Information


Unique identifier OMICS_02315
Name NormqPCR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
License GNU Lesser General Public License version 3.0
Computer skills Advanced
Version 1.26.0
Stability Stable
Requirements
methods, stats, RColorBrewer, Biobase, R(>=2.14.0), qpcR, ReadqPCR
Maintained Yes

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Publication for NormqPCR

NormqPCR in publications

 (27)
PMCID: 5846725
PMID: 29529054
DOI: 10.1371/journal.pone.0193076

[…] [], poplar [–],eucalyptus [,], and teak []. to determine which reference gene is best for transcript normalization in a given subset of biological samples, statistical algorithms such as reffinder, normqpcr, refgenes, oliver, genorm, bestkeeper, and normfinder have been developed (https://omictools.com/reference-genes-category) [,]., we used statistical and graphical methods to compare […]

PMCID: 5841787
PMID: 29513715
DOI: 10.1371/journal.pone.0193727

[…] different genes to be the most stable across different developmental stages (eggs, adults) and stressors (salinity shock, handling—and density stress) after assessment with the genorm method in the normqpcr r package []., to ensure the identity of the primer product sequences originated from the a. tonsa transcriptome, conventional pcr were used to generate pcr products and submitted […]

PMCID: 5835576
PMID: 29402726
DOI: 10.1016/j.ebiom.2018.01.031

[…] the commonly used housekeeping (hk) genes like β-actin, α-tubulin, gusb and gapdh were measured. in order to select the suitable hk genes in our cohort, we performed genorm analysis using r package “normqpcr” (). as a result, the m values of β-actin, α-tubulin, gusb and gapdh were 0.11, 0.08, 0.08 and 0.07, respectively. thus, gapdh was selected as the most stable hk gene owing to the lowest […]

PMCID: 5622125
PMID: 28963513
DOI: 10.1038/s41598-017-10861-6

[…] 1.2.1 (applied biosystems). measured cq values were corrected for primer efficiency cqe = cq(log(1+e)/log2) and further analyses were performed using the r packages readqpcr (version 1.16.0) and normqpcr (version 1.16.0). the geometric mean of the two reference genes was used to normalize the expression of target genes. relative gene expression was calculated using the 2−δδcq method […]

PMCID: 5847100
PMID: 28716985
DOI: 10.1136/jim-2017-000457

[…] micrornas for analysis. raw ct expression values were normalized using the ∆ct method, with the top 10 housekeeping micrornas obtained from the genorm algorithm, implemented in the r bioconductor normqpcr package, v.1.20 mrna profiling. mrna profiles were generated using illumina’s whole genome gene expression dasl ht assay. approximately 200 ng of rna from 19 primary ovarian cancer tissue […]


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NormqPCR institution(s)
Institute of Structural and Molecular Biology, University College of London, London, UK

NormqPCR review

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jiang tao

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Desktop
I wang to install the ReadqPCR, "package ‘ReadqPCR’ is not available (for R version 3.4.3)". So disappointed.