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Enumerates clustered circular objects such as bacterial colonies. OpenCFU can handle digital pictures as well as live stream from a video device/webcam. This software is cross-platform, fast, reliable and allows the user to implement intuitive filters. It was shown to be very accurate and, with high definition pictures, did not generate more errors than the average human error. Finally, OpenCFU provides postprocessing filters to eliminate detected objects according to their intensity and colour similarity. This makes the method even more robust since it can be used to exclude circular artefacts such as contaminant bacteria.