Searches the ever-growing arthropod transcriptome databases so that pest-specific RNAi sequences can be identified. The web tool OfftargetFinder can be used to identify stretches of nucleotides that do not contain 21mer matches with a library of over 100 arthropod transcriptomes. It can therefore be used to design RNAi pesticides likely to have a narrow spectrum of affected species. This will help technology developers finesse the design of RNAi sequences and suggests which non-target species should be assessed in the risk assessment process.
The Bio21 Institute, the University of Melbourne, Australia; School of Biosciences, the University of Melbourne, Australia; CSIRO National Facilities and Collections, Canberra ACT, Australia; Faculty of Veterinary and Agricultural Sciences, the University of Melbourne, Australia; CSIRO Land and Water Flagship, Canberra ACT, Australia
OfftargetFinder funding source(s)
This work has been supported by the Australia India Strategic Research Fund, Grand Challenge Scheme, Grant Number GCF010009.