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Provides useful predictions of oligonucleotide properties under various experimental conditions. The OligoAnalyzer is the central calculator where various kinds of information about an oligonucleotide sequence can be predicted. Selection of the analyze button results in the physical properties of the oligonucleotide, such as a complementary sequence, oligonucleotide length, content of G and C bases, melting temperature, extinction coefficient at 260 nm and molecular weight. OligoAnalyzer was designed to position locked nucleic acids (LNA) modifications within a sequence, so that the desired melting temperature of the duplex sequence is achieved.

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OligoAnalyzer classification

OligoAnalyzer specifications

Unique identifier:
Restrictions to use:
Computer skills:
Web user interface
Input data:
A nucleotide sequence and conditions (i.e. the concentrations of DNA, Na+, K+, Mg2+) and deoxynucleoside triphosphates.

OligoAnalyzer support


  • OligoAlyzer Team <>
  • Richard Owczarzy <>

Additional information


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Department of Molecular Genetics and Biophysics, Coralville, IA, USA; Department of Bioinformatics, Coralville, IA, USA; Department of Information Services, Integrated DNA Technologies Inc; Coralville, IA, USA

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.