OligoEvaluator statistics

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OligoEvaluator specifications


Unique identifier OMICS_02353
Name OligoEvaluator
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

OligoEvaluator in publications

PMCID: 5622086
PMID: 28963492
DOI: 10.1038/s41598-017-12789-3

[…] tertiary structure of bama was predicted by swiss-model., chromosomal dna of a. baumannii atcc 19606 was isolated and used as template for pcr. primers were designed by online tool ‘oligoevaluator™’ for bama amplification having bamhi and xhoi restriction sites in forward (5′- ataggatcctgtggtggaggaagtt-3′) and reverse (5′- tcactcgagttattttgtcttaatttgataacaat-3) primers […]

PMCID: 5370143
PMID: 28350819
DOI: 10.1371/journal.pone.0174590

[…] respectively (). in silico examination of the probes and primers was performed using the oligo evaluator feature within the realtimedesign software (https://www.biosearchtech.com/probeity/design/oligoevaluator.aspx)., *ns = non-synonymous, s = synonymous, for the taqman pcr assay, dna extracted from pure laboratory stocks of csw-1 and v1-99 iltv were used as control samples using three […]

PMCID: 5259778
PMID: 28117454
DOI: 10.1038/srep41105

[…] from antennal rna using the smarter kit (clontech, mountain view, ca, usa). primer sequences were designed using existing contig data as reference, and thermodynamical features were checked by oligoevaluator (sigma genosys, http://www.oligoevaluator.com/). putative oligodimerization was checked by oligoanalyzer 3.1 (integrated dna technologies, http://eu.idtdna.com/calc/analyzer), […]

PMCID: 5105840
PMID: 27591749
DOI: 10.1534/genetics.116.188151

[…] synthesized from 1 µg of total rna, using oligo dt and superscript reverse transcriptase ii (invitrogen). primers for pcr were designed using the online program primer 3 (v.0.4.0) and verified with oligoevaluator (sigma chemical, st. louis, mo) to discard dimer structure formation. pcr efficiencies of the target and reference genes were determined by generating standard curves, based on serial […]

PMCID: 4986182
PMID: 27528376
DOI: 10.1186/s13287-016-0373-5

[…] sequences were obtained from ncbi unigene (http://www.ncbi.nlm.nih.gov/unigene). primers were designed using ncbi primer blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast) and optimized by oligoevaluator™ (http://www.sigmaaldrich.com/technical-documents/articles/biology/oligo-evaluator.html) or tm calculator […]

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