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OligoWiz specifications

Information


Unique identifier OMICS_00831
Name OligoWiz
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline OligoWiz Team <>

Publications for OligoWiz

OligoWiz in publications

 (28)
PMCID: 5390290
PMID: 28406170
DOI: 10.1038/srep46409

[…] a good rna integrity. the ratios 260/230 and 260/280 were greater than 1, 8., a pangenomic 4 × 72 k nimblegen microarray (roche diagnostics, france) was designed. for this purpose, we used the oligowiz software and a reference pangenome composed of a representative sequence for each gene cluster to build a repertoire of possible isothermal probes of length between 45 nt and 65 nt. […]

PMCID: 5382490
PMID: 28381285
DOI: 10.1186/s12985-017-0738-0

[…] aligned by using clustal w algorithm in mega 6.0 software by method of progressive multiplex alignment. oligonucleotide probes with optimal physicochemical characteristics were selected with use of oligowiz 2.1 and picky 2.20 programs., specificity of the chosen oligonucleotide primers and probes was tested with use of blast (basic local alignment search tool) program that could compare […]

PMCID: 5100562
PMID: 27402158
DOI: 10.1093/nar/gkw603

[…] 10 mm dntps, his6 mmlv rt (or commercial alternative)), samples were incubated for 2 h at 42°c and then stored at −20°c., all forward and reverse primers for quantitative pcr were designed using oligowiz 2.0 (http://www.cbs.dtu.dk/services/oligowiz/) (). representative subtelomeric genes were selected that met the following criteria: they were within 50 kb from the end of a chromosome; […]

PMCID: 4991868
PMID: 27602258
DOI: 10.7717/peerj.2216

[…] of the ppcp1 plasmid of the y. pestis genome (cp000310.1) was obtained from the ncbi database for microarray probe design. probes were designed using two publicly available software packages: oligowiz (http://www.cbs.dtu.dk/services/oligowiz/) and unique probe selector (http://array.iis.sinica.edu.tw/ups/). all probes were checked for suitability using an in silico blast analysis. […]

PMCID: 4585701
PMID: 26388459
DOI: 10.1038/srep14199

[…] was isolated, and the clone was designated as tm29., custom single-channel dna microarrays for l. lactis mg1363 with probes for all annotated genes in the published sequence were designed using oligowiz and chips were ordered from agilent., cells were cultivated in bottles under the same condition as the growth experiment. four independent biological samples were harvested […]


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OligoWiz institution(s)
Center for Biological Sequence Analysis, Technical University of Denmark, Kgs, Lyngby, Denmark

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