Facilitates analysis of microarrays and miRNA/RNA-seq data on laptops. oneChannelGUI can be used for quality control, normalization, filtering, statistical validation and data mining for single channel microarrays. It offers a comprehensive microarray analysis for Affymetrix 3′ (IVT) expression arrays as well as for the new generation of whole transcript arrays: human/mouse/rat exon 1.0 ST and human gene 1.0 ST arrays. oneChannelGUI inherits the core affylmGUI functionalities and permits a wider range of analysis allowing biologists to choose among different criteria and algorithms in order to analyze their data. It is a didactical tool since it could be used to introduce young life scientists to the use and interpretation of microarray data. For this purpose various data sets and exercises are available at the oneChannelGUI web site.