OpenSPIM specifications

Information


Unique identifier OMICS_10786
Name OpenSPIM
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java
Computer skills Medium
Stability Stable
Maintained Yes

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Publication for OpenSPIM

OpenSPIM in publications

 (15)
PMCID: 5606987
PMID: 28931809
DOI: 10.1038/s41467-017-00514-7

[…] the in-focus fluorescence. an example hilo reconstruction is shown in supplementary fig. ., all samples were mounted to a custom pipette tip mount, inspired by previous work from zeiss microscopy, openspim, and openspin for our specific imaging chamber and rssm, . the end of the pipette tip was plugged with rims and 1% agar and a small amount of cyanoacrylate glue holds the tissue specimen […]

PMCID: 5529444
PMID: 28747660
DOI: 10.1038/s41598-017-06646-6

[…] pixels, 4 × 4 binning, 40 ms exposure, andor, belfast, northern ireland) to capture images. the camera and rotational axis (picard instruments, albion, usa) were controlled though μmanager and the openspim plugin. the point spread function (psf) of our system was determined by performing z-stacks of 50 × 0.5 µm fluorescent beads, embedded in 1% agarose within a glass capillary. images […]

PMCID: 5940348
PMID: 29750130
DOI: 10.1038/s41551-017-0084

[…] when viewed with moderate- to high-na microscope objectives. finally, light-sheet microscopy systems can be simple and relatively inexpensive to build, as demonstrated by us and others (e.g., the openspim project) []., in summary, camera-based tissue-microscopy approaches (e.g., sim and muse) are fast, but generally provide poor imaging depth (background suppression), which limits […]

PMCID: 5473845
PMID: 28623366
DOI: 10.1038/s41598-017-03900-9

[…] a cryostat, fluorescent microscope and personal computer, would cost approximately us$115250 (supplementary table ), which is more expensive than self-made light sheet microscope (us$57000; openspim), and less expensive than commercial light sheet microscope (us$260000), µct (us$200000–1 million), mri ($1 million per tesla of magnetic field), and sem for atum specimens […]

PMCID: 5354410
PMID: 28301563
DOI: 10.1371/journal.pone.0173879

[…] sharing these implementations the hope is that downstream users can take advantage of these materials without having to reinvent the fiber launch, so to speak. in the same vein as projects such as openspim [], this provides a starting point for others who are encouraged to share their own improvements and modifications., two generations of this project are presented. the first, referred […]


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OpenSPIM institution(s)
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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