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Optimal Paired-End Read Assembler Opera

Alternative name: OPERA-LG

A scalable, exact algorithm for the scaffold assembly of large, repeat-rich genomes, with consistent improvement over state-of-the-art programs for scaffold correctness and contiguity. OPERA provides a rigorous framework for scaffolding of repetitive sequences and a systematic approach for combining data from different second-generation (Illumina, Ion Torrent) and third-generation (PacBio, ONT) sequencing technologies. OPERA efficiently scaffolds large genomes with provable scaffold properties, providing an avenue for systematic augmentation and improvement of 1000s of existing draft eukaryotic genome assemblies.

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Opera classification

Opera specifications

Software type:
Restrictions to use:
Output data:
Operating system:
Command line interface
Input data:
Paired-end/mate-pair reads
Biological technology:
Illumina, Life Technologies, Pacific Biosciences, Roche, Oxford Nanopore
Computer skills:

Opera distribution


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Opera support


  • Song Gao <>


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NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore

Link to literature

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