Determines the baseline by reconstructing the loglinear phase downward from the early plateau phase of the polymerase chain reaction (PCR). LinRegPCR is designed for improving reproducible PCR efficiency values. It requires a sufficiently large baseline-to-plateau ratio as well as a low observation noise. This tool identifies the variables of interest and enables the analysis of the significance of unknown variables affecting the analysis.
Identifies and corrects errors in short read sequencing data. PEC uses an in silico, barcode-free strategy to exploit the redundancy that arises when multiple polymerase chain reaction (PCR) amplicons are derived from the same initial DNA fragment. This software fits to applications in which many cycles of PCR are used to generate sufficient DNA for sequencing from small amounts of starting material such as circulating tumor DNA (ctDNA) analysis.