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P-SAMS specifications

Information


Unique identifier OMICS_10138
Name P-SAMS
Alternative name Plant Small RNA Maker Site
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Subtools


  • P-SAMS amiRNA Designer
  • P-SAMS syn-tasiRNA Designer

Maintainer


  • person_outline Noah Fahlgren

Publication for Plant Small RNA Maker Site

P-SAMS citations

 (3)
library_books

MicroRNA Mediated Gene Silencing in Plant Defense and Viral Counter Defense

2017
Front Microbiol
PMCID: 5611411
PMID: 28979248
DOI: 10.3389/fmicb.2017.01801

[…] nserved RNA motifs in the viral genome (). In addition, polycistronic amiRNA-mediated resistance to WSMV was successfully and efficiently applied in wheat and barley, respectively (; ). Recently, the Plant Small RNA Maker Site (P-SAMS) tool1, which serves as a high-throughput platform for the high efficiency design of amiRNA and synthetic trans-acting small interfering RNAs (syn-tasiRNA), has been […]

library_books

Gene Overexpression Resources in Cereals for Functional Genomics and Discovery of Useful Genes

2016
Front Plant Sci
PMCID: 5030214
PMID: 27708649
DOI: 10.3389/fpls.2016.01359

[…] ch the distal stem–loop sequence (16 bp) was replaced with that (31 bp) of Arabidopsis MIR390a (AtMIR390a), and induced effective silencing of individual target genes. In combination with a web tool, Plant Small RNA Maker Site (P-SAMS), for automated design of amiRNAs (), binary vectors carrying the OsMIR390-AtL precursor enable direct cloning of various amiRNAs and could be used for generating la […]

call_split

Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors

2015
Plant J
PMCID: 4464980
PMID: 25809382
DOI: 10.1111/tpj.12835
call_split See protocol

[…] s and Weigel, ; Liang et al., ; Carbonell et al., ). Sequences of both the amiRNAs expressed in Brachypodium and the oligonucleotides used for cloning in OsMIR390‐B/c vectors, were designed with the ‘P‐SAMS amiRNA Designer’ tool (http://p-sams.carringtonlab.org). The predicted targets for all the amiRNAs used in this study are shown in Table S8.The generation of constructs to express amiRNAs from […]

Citations

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P-SAMS institution(s)
Donald Danforth Plant Science Center, St Louis, MO, USA
P-SAMS funding source(s)
This work was supported by the National Institutes of Health [grant number AI043288]; the National Science Foundation [grants numbers MCB-1231726, MCB-1330562]; and the United States Department of Agriculture [fellowship number MOW-2012-01361].

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