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P-SAMS specifications


Unique identifier OMICS_10138
Alternative name Plant Small RNA Maker Site
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Noah Fahlgren <>

Publication for Plant Small RNA Maker Site

P-SAMS in publications

PMCID: 5611411
PMID: 28979248
DOI: 10.3389/fmicb.2017.01801

[…] rna motifs in the viral genome (). in addition, polycistronic amirna-mediated resistance to wsmv was successfully and efficiently applied in wheat and barley, respectively (; ). recently, the plant small rna maker site (p-sams) tool1, which serves as a high-throughput platform for the high efficiency design of amirna and synthetic trans-acting small interfering rnas (syn-tasirna), […]

PMCID: 5030214
PMID: 27708649
DOI: 10.3389/fpls.2016.01359

[…] the distal stem–loop sequence (16 bp) was replaced with that (31 bp) of arabidopsis mir390a (atmir390a), and induced effective silencing of individual target genes. in combination with a web tool, plant small rna maker site (p-sams), for automated design of amirnas (), binary vectors carrying the osmir390-atl precursor enable direct cloning of various amirnas and could be used for generating […]

PMCID: 4464980
PMID: 25809382
DOI: 10.1111/tpj.12835

[…] and from authentic osmir390 precursors (figure a). the sequences for amir‐bdbri1, amir‐bdcad1, amir‐bdcao and amir‐bdspl11 (figure s5) were designed using the ‘p‐sams amirna designer’ tool (http://p-sams.carringtonlab.org). plants expressing 35s:gus were used as negative controls. phenotypes of transgenic plants, amirna accumulation, processing of amirna precursors, and target transcript […]

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P-SAMS institution(s)
Donald Danforth Plant Science Center, St Louis, MO, USA
P-SAMS funding source(s)
This work was supported by the National Institutes of Health [grant number AI043288]; the National Science Foundation [grants numbers MCB-1231726, MCB-1330562]; and the United States Department of Agriculture [fellowship number MOW-2012-01361].

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