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PAGIT specifications


Unique identifier OMICS_23172
Alternative name Post-Assembly Genome-Improvement Toolkit
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
License GNU General Public License version 3.0
Computer skills Advanced
Stability Stable
Maintained Yes


No version available


  • person_outline Thomas Otto

Additional information

The software can be downloaded via a Virtual Machine.

Publication for Post-Assembly Genome-Improvement Toolkit

PAGIT citations


Complete Genome Sequence of Agrobacterium tumefaciens 1D1609

Genome Announc
PMCID: 5908930
PMID: 29674541
DOI: 10.1128/genomeA.00253-18

[…] 11,564,340 reads) and one mate pair library (∼4,100-bp insert, 8,219,766 reads). the de novo assembly was performed using allpaths-lg (). the initial draft assembly was iteratively improved using pagit (). in each iteration, the illumina reads were mapped to the assembly using burrows-wheeler aligner (bwa) (), programmatically checked using samtools (), and visually inspected using […]


Complete Genome Sequence of Spiroplasma floricola 23 6T (ATCC 29989), a Bacterium Isolated from a Tulip Tree (Liriodendron tulipifera L.)

Genome Announc
PMCID: 5908944
PMID: 29674553
DOI: 10.1128/genomeA.00302-18

[…] (∼283-bp insert, ∼416-fold coverage) and one mate-pair library (∼4,000-bp insert, ∼476-fold coverage). the initial de novo assembly was performed using allpaths-lg release 52188 (). subsequently, pagit version 1 () was used to assist an iterative process for improving the assembly. for each iteration, the raw reads were mapped to the assembly using bwa version 0.7.12 (), programmatically […]


Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies

PMCID: 5955452
PMID: 29436903
DOI: 10.1080/21505594.2018.1435249

[…] using soapdenovo2 software [] (kmer size of 49). reads with quality less than 30 over 70% of the read were filtered using ngs qc toolkit []. final assembly was generated using abacas and image (pagit) [], using mucor circinelloides cbs277.49 (genbank assembly accession gca_001638945.1) as reference. abacas was run twice, with an intermediate image step to close additional gaps. for running […]


Comparative Genomics and Identification of an Enterotoxin Bearing Pathogenicity Island, SEPI 1/SECI 1, in Staphylococcus epidermidis Pathogenic Strains

PMCID: 5869381
PMID: 29495323
DOI: 10.3390/toxins10030093

[…] petersburg academic university, st petersburg, russia) with 21-33-55-77-97 k-mers. spades output contigs of < 1000 bp or with coverage of <10× were removed. sequences were completed using the post-assembly genome-improvement toolkit from the sanger institute []. the final sequence annotation was performed with the ncbi genome annotation pipeline. the unique chromosomes for each sequence […]


Multiple Roots of Fruiting Body Formation in Amoebozoa

Genome Biol Evol
PMCID: 5804921
PMID: 29378020
DOI: 10.1093/gbe/evy011

[…] and blastx for fungi). if a contig had a higher similarity to known bacterial or fungal sequences than to any amoebozoa genome, it was excluded from the analysis. further gap closure was done with pagit (; last accessed january 31, 2018) using default values. for assembly of the mitochondrial genome, we extended the contig using sspace (). […]


Whole genome sequencing of the monomorphic pathogen Mycobacterium bovis reveals local differentiation of cattle clinical isolates

BMC Genomics
PMCID: 5748942
PMID: 29291727
DOI: 10.1186/s12864-017-4249-6

[…] spades (v3.6.1) [], using the previous velvet assembly as known data and changing the set of trial k-mers in each. all three resulting assemblies were integrated with the software cisa (v1.3) []., pagit toolkit [] was used to further improve the quality of the assembly by closing gaps on scaffolds, correcting base errors and generate an annotation of cds based on the reference genome […]

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PAGIT institution(s)
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais Campus, Aberystwyth, UK; WeatherallInstitute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
PAGIT funding source(s)
Supported by the European Union 7th framework EVIMalaR and by the Wellcome Trust [Grant number: 098051].

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