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pCLAMP specifications

Information


Unique identifier OMICS_25410
Name pCLAMP
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
Computer skills Medium
Version 11
Stability Stable
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline pCLAMP Team

pCLAMP citations

 (4303)
library_books

Graphene biointerfaces for optical stimulation of cells

2018
Sci Adv
PMCID: 5959318
DOI: 10.1126/sciadv.aat0351

[…] filled with a solution consisting of 150 mM KCl, 5 mM NaCl, 5 mM MgATP, 10 mM Hepes, 5 mM EGTA (pH 7.2). All recordings were acquired using a Digidata 1322 interface, an Axopatch 200B amplifier, and pClamp software (Molecular Devices). The data were digitally sampled at 10 kHz and filtered using an eight-pole Bessel analog low-pass filter at 2-kHz cutoff frequency. […]

library_books

Altered Cerebellar Short Term Plasticity but No Change in Postsynaptic AMPA Type Glutamate Receptors in a Mouse Model of Juvenile Batten Disease

2018
eNeuro
PMCID: 5956745
PMID: 29780879
DOI: 10.1523/ENEURO.0387-17.2018

[…] (500 μM, Sigma) was added to this intracellular solution immediately before each recording session.Currents were recorded at 22–26°C using an Axopatch 1D or Axopatch 200B amplifier and acquired using pClamp10 and a Digidata 1200 interface (Molecular Devices). Series resistance and input capacitance were read directly from the amplifier settings used to minimize the current responses to 5-mV hyperp […]

library_books

LRRC8/VRAC anion channels enhance β cell glucose sensing and insulin secretion

2018
Nat Commun
PMCID: 5958052
PMID: 29773801
DOI: 10.1038/s41467-018-04353-y

[…] ntrations (220 mOsm). All recordings were performed at room temperature. We used an EPC-10 patch-clamp amplifier controlled with the PatchMaster software (HEKA). Data were analyzed using the software pClamp 10.6 (Molecular Devices). Patch pipettes were pulled to a resistance of 2–5 MΩ. […]

library_books

A Drosophila Model of Essential Tremor

2018
Sci Rep
PMCID: 5955955
PMID: 29769701
DOI: 10.1038/s41598-018-25949-w

[…] rnal solution as described previously,. Glass pipettes (8–15 MΩ) were pulled using a Sutter P-1000 puller and filled with internal solution. The resulting signal was then monitored and recorded using pClamp10 Clampex software and pipette offsets were zeroed prior to cell contact with the pipette capacitance being compensated upon contact. Subsequent signals were passed through a 10 kHz low-pass Be […]

library_books

Kv1.3 activity perturbs the homeostatic properties of astrocytes in glioma

2018
Sci Rep
PMCID: 5955950
PMID: 29769580
DOI: 10.1038/s41598-018-25940-5

[…] 140 KCl, 13.4 NaCl, 0.5 CaCl2, 1 MgCl2, 5 EGTA, 10 Hepes, and 3 Mg-ATP, 0.3 Na3-GTP (280–285 mOsm, pH 7.2). Signals were acquired (sampling 10 kHz, low-pass filtered 2 kHz) with DigiData-1440A using pCLAMP-v10 software (Molecular Devices, USA). Membrane capacitance was estimated as the total charge (i.e., the current integral, Qstep) mobilized in each cell by a 10 mV hyperpolarizing step (Vstep): […]

library_books

Phasic Stimulation of Midbrain Dopamine Neuron Activity Reduces Salt Consumption

2018
eNeuro
PMCID: 5952649
PMID: 29766048
DOI: 10.1523/ENEURO.0064-18.2018

[…] pacitance (Cm) was measured under voltage clamp at −50 mV using a hyperpolarizing 10 mV, 250-ms step. Cm was measured from the change in membrane charge taken from the integrated capacity transients (pClamp, Molecular Devices). All potentials cited here have not been corrected for liquid junction potentials (estimated using pClamp calculator as 9.2 mV). Slices were incubated in drug cocktails for […]

Citations

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pCLAMP institution(s)
Molecular Devices, LLC, San Jose,CA, USA

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