pcrEfficiency specifications

Information


Unique identifier OMICS_02374
Name pcrEfficiency
Interface Web user interface
Restrictions to use None
License GNU General Public License version 2.0
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Izaskun Mallo <>

Publication for pcrEfficiency

pcrEfficiency in publications

 (6)
PMCID: 5813328
PMID: 29444635
DOI: 10.1186/s12870-018-1244-2

[…] (cvc). circadian clock related genes were gigantea (gi), timing of cab expression1 (toc1), late elongated hypocotyl (lhy), and early flowering 3(elf3). primers were designed using the software pcrefficiency (http://srvgen.upct.es/efficiency.html) as described previously [] (additional file : table s1). primers were tested for stable, single and clear amplification products by end-point pcr […]

PMCID: 5065972
PMID: 27799920
DOI: 10.3389/fmicb.2016.01578

[…] removed with rnase-free dnase i (qiagen) and the remaining rna was purified using a qiagen's rneasy column. primers were designed to have the same melting temperature and pcr efficiency using the pcrefficiency open source tool (http://srvgen.upct.es/efficiency.html; mallona et al., ). complementary strand synthesis was performed in a 20 μl reaction mixture containing 1 μg of total rna, 10 […]

PMCID: 4773603
PMID: 26973686
DOI: 10.3389/fpls.2016.00245

[…] genes for normalization were described previously (belda-palazón et al., ). the primers used for qpcr not reported previously are indicated in table and were designed using the web tool pcrefficiency (mallona et al., ). relative gene expression values and statistical analysis was performed using the rest 2009 software program (pfaffl et al., ) available from qiagen […]

PMCID: 4827641
PMID: 27077046
DOI: 10.1016/j.bdq.2015.12.002

[…] and cq value of reactions with 104 target rna copies/reaction, which is a measure for the reaction velocity. the abstracted target value was, thus, calculated as follows:(1)target  value=a×r2+b×pcrefficiency+c×signal   increased×cqvalue  at  104copies/reaction, coefficients a–d were used to balance the performance characteristic value influence on the target value. coefficient values […]

PMCID: 5065590
PMID: 26525392
DOI: 10.1007/s00204-015-1621-7

[…] \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\text{pcr}}\;{\text{efficiency}} = 10^{{( - 1/{\text{slope}})}} {-}1$$\end{document}pcrefficiency=10(-1/slope)-1 fig. 2 , the optimum value for the primer efficiency would be 1.00 (100 %) if the template is doubled within every pcr cycle (bustin et al. ). even though a 200-fold […]


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pcrEfficiency institution(s)
Genetics, Institute of Plant Biotechnology (IBV), Technical University of Cartagena (UPCT), Campus Muralla del Mar, Cartagena, Spain

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