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pcrEfficiency specifications


Unique identifier OMICS_02374
Name pcrEfficiency
Interface Web user interface
Restrictions to use None
License GNU General Public License version 2.0
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Izaskun Mallo

Publication for pcrEfficiency

pcrEfficiency citations


Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata)

BMC Plant Biol
PMCID: 5813328
PMID: 29444635
DOI: 10.1186/s12870-018-1244-2
call_split See protocol

[…] ICILIN (CVC). Circadian clock related genes were GIGANTEA (GI), TIMING OF CAB EXPRESSION1 (TOC1), LATE ELONGATED HYPOCOTYL (LHY), and EARLY FLOWERING 3(ELF3). Primers were designed using the software PCRefficiency (http://srvgen.upct.es/efficiency.html) as described previously [] (Additional file : Table S1). Primers were tested for stable, single and clear amplification products by end-point PCR […]


Genetic Identification of a PilT Motor in Geobacter sulfurreducens Reveals a Role for Pilus Retraction in Extracellular Electron Transfer

Front Microbiol
PMCID: 5065972
PMID: 27799920
DOI: 10.3389/fmicb.2016.01578

[…] was removed with RNase-free DNase I (QIAGEN) and the remaining RNA was purified using a QIAGEN's RNeasy column. Primers were designed to have the same melting temperature and PCR efficiency using the pcrEfficiency open source tool (http://srvgen.upct.es/efficiency.html; Mallona et al., ). Complementary strand synthesis was performed in a 20 μl reaction mixture containing 1 μg of total RNA, 10 pmol […]


Relevance of the Axis Spermidine/eIF5A for Plant Growth and Development

Front Plant Sci
PMCID: 4773603
PMID: 26973686
DOI: 10.3389/fpls.2016.00245

[…] housekeeping genes for normalization were described previously (Belda-Palazón et al., ). The primers used for qPCR not reported previously are indicated in Table and were designed using the web tool pcrEfficiency (Mallona et al., ). Relative gene expression values and statistical analysis was performed using the REST 2009 software program (Pfaffl et al., ) available from Qiagen (http://www.qiagen […]


Sequences within Both the 5′ UTR and Gag Are Required for Optimal In Vivo Packaging and Propagation of Mouse Mammary Tumor Virus (MMTV) Genomic RNA

PLoS One
PMCID: 3473059
PMID: 23077548
DOI: 10.1371/journal.pone.0047088

[…] ecificity, reproducibility, and amplification efficiency of the assay performance. Further validation of the PCR amplification efficiency was conducted by testing the assay using the online web tool “pcrEfficiency” that confirmed it to be over 100%. As an endogenous control, the optimized ß-actin MGB VIC-labeled assay (no 4326315E with limited primer concentration) from ABI was used (Applied Bios […]


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pcrEfficiency institution(s)
Genetics, Institute of Plant Biotechnology (IBV), Technical University of Cartagena (UPCT), Campus Muralla del Mar, Cartagena, Spain

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