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PCRP specifications

Information


Unique identifier OMICS_16808
Name PCRP
Alternative name Protein Capture Reagents Program
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Not allowed
Maintained Yes

Maintainer


  • person_outline Seth Blackshaw

Publication for Protein Capture Reagents Program

PCRP citations

 (7)
library_books

Transitional changes in the CRP structure lead to the exposure of proinflammatory binding sites

2017
Nat Commun
PMCID: 5264208
PMID: 28112148
DOI: 10.1038/ncomms14188

[…] default values) to relieve any steric conflicts that may have arisen during the docking or modelling process. The pentameric pCRP* model was constructed by manually moving the individual subunits of pCRP apart until the interacting side chains were >4 Å apart () or the globular head of complement C1q (PDB ID: 1PK6; ref. ) was able to dock inside the inner annular void, bringing the known interact […]

library_books

Complement factor H binding of monomeric C reactive protein downregulates proinflammatory activity and is impaired with at risk polymorphic CFH variants

2016
Sci Rep
PMCID: 4785391
PMID: 26961257
DOI: 10.1038/srep22889

[…] es showing mCRP-induced IL-8 and CCL2 upregulation in endothelial cells and neutrophils. By contrast, our results are at odds with a previous report of increased IL-8 expression on ARPE-19 cells upon pCRP stimulation. In this regard, we notice that Wang and colleagues used higher, supraphysiological concentrations of pCRP (up to 100 μg/mL), well above those associated with AMD risk.In our hands, F […]

library_books

The in vitro and in vivo effects of constitutive light expression on a bioluminescent strain of the mouse enteropathogen Citrobacter rodentium

2016
PeerJ
PMCID: 4924136
PMID: 27366640
DOI: 10.7717/peerj.2130

[…] dentium, reads were also mapped to the sequenced plasmids pCROD1 (Genbank accession number FN543503.1), pCROD2 (Genbank accession number FN543504.1), pCROD3 (Genbank accession number FN543505.1), and pCRP3 (Genbank accession number NC_003114). […]

library_books

The Binding of Monomeric C Reactive Protein (mCRP) to Integrins αvβ3 and α4β1 Is Related to Its Pro Inflammatory Action

2014
PLoS One
PMCID: 3973595
PMID: 24695572
DOI: 10.1371/journal.pone.0093738

[…] Chemotaxis was measured in modified Boyden chambers (Transwells). 50 μg/ml mCRP or pCRP in 600 μl RPMI 1640 medium was placed in the lower chamber, and U937 cells (5×105 cells per well) were placed in the upper chamber. In some case, U937 cells were preincubated with antibodies (25 […]

library_books

Transposon and Deletion Mutagenesis of Genes Involved in Perchlorate Reduction in Azospira suillum PS

2013
MBio
PMCID: 3884062
PMID: 24381299
DOI: 10.1128/mBio.00769-13

[…] n). The digested products were then ligated into the vector using T4 ligase (NEB). The broad-host-range plasmid pBBR1MCS2 was used as the backbone for the complementation vectors (). For three genes (pcrP, pcrS, and pcrR), pBBR1MCS2 and the amplified gene with its promoter were digested, purified, and ligated as described above. For the pcrABCD and cld complementation vectors, the pcrA promoter wa […]

library_books

Opposing effects of monomeric and pentameric C reactive protein on endothelial progenitor cells

2011
PMCID: 3149664
PMID: 21562922
DOI: 10.1007/s00395-011-0191-y

[…] Total RNA was extracted from 2 × 106 EPCs after 72 h of culture in EGM-2 medium in the presence or absence of mCRP (1 μg/ml), pCRP (5 μg/ml) or PBS. The RNA was obtained using Qiagen® RNeasy protect mini kit™ following the manufacturer’s instructions. RNA concentration and integrity was analysed by NanoDrop (Thermo Fischer S […]

Citations

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PCRP institution(s)
Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MA, USA; Department of Neurology, Johns Hopkins University, Baltimore, MA, USA; Department of Ophthalmology, Johns Hopkins University, Baltimore, MA, USA; Center for Human Systems Biology, Johns Hopkins University, Baltimore, MA, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MA, USA; CDI Laboratories, Guanajibo Research and Innovation Park, Mayaguez, PR, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MA, USA; Department of Molecular Biology and Biochemistry, Rutgers University, New Brunswick, NJ, USA; Center for Advanced Biotechnology and Medicine, Piscataway, NJ, USA; National Human Genome Research Institute, Bethesda, MA, USA; National Institutes of Health, Bethesda, MA, USA; Division of Scientific Programs, National Institute on Deafness and Other Communications Disorders, Bethesda, MA, USA; Office of Strategic Coordination, Office of the Director, National Institutes of Health, Bethesda, MA, USA; National Institute of Neurological Disorders and Stroke, Bethesda, MA, USA; Advanced Biomedical Computing Center, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MA, USA; Department of Pharmacology, Johns Hopkins University, Baltimore, MA, USA; Neuroscience Center, Bethesda, MA, USA
PCRP funding source(s)
Supported by NIH grants U54HG006434, U01DC011485, and U54HG006436.

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