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PCRTiler specifications

Information


Unique identifier OMICS_20455
Name PCRTiler
Software type Pipeline/Workflow
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
License GNU General Public License version 3.0
Computer skills Medium
Version 1.42
Stability Stable
Source code URL http://pcrtiler.genap.ca/PCRTiler/PCRTiler-v1.42-standalone-src.tgz
Maintained Yes

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Versioning


No version available

Maintainer


  • person_outline Alain Gervais

Information


Unique identifier OMICS_20455
Name PCRTiler
Interface Web user interface
Restrictions to use None
License GNU General Public License version 3.0
Computer skills Basic
Stability Stable
Maintained Yes

Download


download.png

Maintainer


  • person_outline Alain Gervais

Publication for PCRTiler

PCRTiler citations

 (3)
call_split

BRDT is an essential epigenetic regulator for proper chromatin organization, silencing of sex chromosomes and crossover formation in male meiosis

2018
PLoS Genet
PMCID: 5841650
PMID: 29513658
DOI: 10.1371/journal.pgen.1007209
call_split See protocol

[…] Tiled primer pairs covering the region comprising from 166,425 to 166,428 kb in the X chromosome, were designed using the PCRTiler software []. We obtained 50 pairs of primers with an overlap of ~50 kb that yield amplicons of ~100 kb. Primer sets are provided in . Primer pairs were tested and those that produced multiple […]

library_books

Nucleosomes Are Stably Evicted from Enhancers but Not Promoters upon Induction of Certain Pro Inflammatory Genes in Mouse Macrophages

2014
PLoS One
PMCID: 3976374
PMID: 24705533
DOI: 10.1371/journal.pone.0093971

[…] DNA and cDNA was quantified on a Lightcycler 480 (Roche) as described with the following modifications. Primers were designed using the program PCRtiler . To verify that only a single amplicon was produced by each primer pair and no primer dimers were formed a Tm-curve was performed as a quality control for each primer pair at the end of each […]

call_split

Genomic Analysis of a Novel Spontaneous Albino C57BL/6N Mouse Strain

2013
PMCID: 3799019
PMID: 23620107
DOI: 10.1002/dvg.22398
call_split See protocol

[…] A tiling path of 26 amplicons covering ∼24 kb with minimal overlap was designed using PCRTiler software. Reactions were performed on a selection of albino and wildtype control mice. Amplification conditions were 94°C 5 min, followed by 35 cycles of 94°C 30 sec, 58°C 30 sec, 72°C 1 min […]

Citations

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PCRTiler institution(s)
Departement de Biologie, Universite de Sherbrooke , Sherbrooke, QC, Canada
PCRTiler funding source(s)
Supported by the Fonds Quebecois de la Recherche sur la Nature et les Technologies; Canada Research Chair on Mechanisms of Gene Transcription; Canadian Institute of Health Research.

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