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PCRTiler specifications

Information


Unique identifier OMICS_20455
Name PCRTiler
Software type Pipeline/Workflow
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
License GNU General Public License version 3.0
Computer skills Medium
Version 1.42
Stability Stable
Source code URL http://pcrtiler.genap.ca/PCRTiler/PCRTiler-v1.42-standalone-src.tgz
Maintained Yes

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Maintainer


  • person_outline Alain Gervais <>

Information


Unique identifier OMICS_20455
Name PCRTiler
Interface Web user interface
Restrictions to use None
License GNU General Public License version 3.0
Computer skills Basic
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Alain Gervais <>

Publication for PCRTiler

PCRTiler in publications

 (3)
PMCID: 5841650
PMID: 29513658
DOI: 10.1371/journal.pgen.1007209

[…] dna fragments was then quantified and used for tiled qpcr analysis., tiled primer pairs covering the region comprising from 166,425 to 166,428 kb in the x chromosome, were designed using the pcrtiler software []. we obtained 50 pairs of primers with an overlap of ~50 kb that yield amplicons of ~100 kb. primer sets are provided in . primer pairs were tested and those that produced […]

PMCID: 3976374
PMID: 24705533
DOI: 10.1371/journal.pone.0093971

[…] at around cycle 20 for the majority of primer pairs., dna and cdna was quantified on a lightcycler 480 (roche) as described with the following modifications. primers were designed using the program pcrtiler . to verify that only a single amplicon was produced by each primer pair and no primer dimers were formed a tm-curve was performed as a quality control for each primer pair at the end […]

PMCID: 3799019
PMID: 23620107
DOI: 10.1002/dvg.22398

[…] could be identified., pcr analysis of the tyrosinase gene (the most likely candidate locus) failed to amplify exons 1 and 2 from albino mouse dna. further analysis of a 24 kb pcr tiling path using pcrtiler () revealed a 14.3 kb deletion 940 bp 5′ of exon 1, including the minimal promoter region (), extending 3.75 kb 3′ of exon 2; in addition, a 2 bp ta insertion was found at the breakpoint (). […]


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PCRTiler institution(s)
Departement de Biologie, Universite de Sherbrooke , Sherbrooke, QC, Canada
PCRTiler funding source(s)
Supported by the Fonds Quebecois de la Recherche sur la Nature et les Technologies; Canada Research Chair on Mechanisms of Gene Transcription; Canadian Institute of Health Research.

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