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PDQuest 2-D specifications

Information


Unique identifier OMICS_02539
Name PDQuest 2-D
Alternative name PDQuest
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained No

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No version available

PDQuest 2-D citations

 (144)
library_books

Proteomic analysis reveals that auxin homeostasis influences the eighth internode length heterosis in maize (Zea mays)

2018
Sci Rep
PMCID: 5940786
PMID: 29739966
DOI: 10.1038/s41598-018-23874-6

[…] ssie brilliant blue G250 and photographed with the PowerLook 2100 XL scanner (UMAX). Protein spots were detected and matched with the default parameters of the “spot detection wizard” function in the PDQuest 8.0 program. The “find spot centers” function was used with default auto-noise smoothing and background subtraction. A Gaussian model was applied to generate a master gel for each image file. […]

library_books

Metabolic and Evolutionary Insights in the Transformation of Diphenylamine by a Pseudomonas putida Strain Unravelled by Genomic, Proteomic, and Transcription Analysis

2018
Front Microbiol
PMCID: 5897751
PMID: 29681895
DOI: 10.3389/fmicb.2018.00676

[…] d then by SDS–PAGE using 12.5% Tris-HCl polyacrylamide gels (Bio-Rad) following standard procedures. 2-D gels were silver stained and scanned with Bio-Rad GS-800 Calibrated Densitometer equipped with PDQuest Advanced 2-D Gel Analysis software (version 8.1, Bio-Rad) as described by Ziogas et al. (). Data were analyzed by one-way ANOVA (P ≤ 0.05) and means were compared using Student's t-test (signi […]

library_books

Blood coagulation abnormalities in multibacillary leprosy patients

2018
PLoS Negl Trop Dis
PMCID: 5863944
PMID: 29565968
DOI: 10.1371/journal.pntd.0006214

[…] Gels were stained with colloidal Coomassie Brilliant Blue G-250 and documented using a GS-800 auto-calibrating imaging densitometer (Bio-Rad). Image analysis was performed using PDQuest software, version 8.0.1 (Bio-Rad). Comparative 2D data were derived from three biological replicates from each clot type (control clot and leprosy clot). The spots were quantified based on the […]

library_books

The rolB plant oncogene affects multiple signaling protein modules related to hormone signaling and plant defense

2018
Sci Rep
PMCID: 5797197
PMID: 29396465
DOI: 10.1038/s41598-018-20694-6

[…] d. On average, 1,500 protein spots were detected on gels of Arabidopsis calli. A local regression model (Loess) was used for normalization of spot intensity. The protein expression was accessed using PDQuest 8.0.1 Advanced software and was presented as mean total intensity of a defined spot in a replicate gel group. Spot quantity is the sum of the intensities of pixels inside the boundary. Fold of […]

library_books

Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre storage Cold Acclimation

2018
Front Plant Sci
PMCID: 5778441
PMID: 29403505
DOI: 10.3389/fpls.2017.02167

[…] The stained gels were scanned using UMAX 2100XL-USB scanner (Bio-Rad, USA) at a resolution of 600 dpi. The gel images were saved in TIF format, and analyzed using PDQuest 2-D analysis software (Version 8.0, Bio-Rad, USA). Three gels with well-separated protein spots of each treatment were used to create “replicate groups” (Chandramouli et al., ; Wang et al., ). […]

library_books

Proteomic analysis of chicken embryo fibroblast cells infected with recombinant H5N1 avian influenza viruses with and without NS1 eIF4GI binding domain

2017
Oncotarget
PMCID: 5823584
PMID: 29492200
DOI: 10.18632/oncotarget.23615

[…] d) control CEFs were prepared and loaded on to 2-DE gels for separation. To compensate for gel electrophoresis variability, we ran three replicate gels for each time point. Based on image analysis by PDQuest software, pairwise comparisons of relative protein abundance were performed among the three groups (rNS1-wt versus control, rNS1-SD30 versus control, and rNS1-wt versus rNS1-SD30). Visual anal […]

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