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PDQuest 2-D specifications


Unique identifier OMICS_02539
Name PDQuest 2-D
Alternative name PDQuest
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained No


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PDQuest 2-D citations


Proteomic analysis reveals that auxin homeostasis influences the eighth internode length heterosis in maize (Zea mays)

Sci Rep
PMCID: 5940786
PMID: 29739966
DOI: 10.1038/s41598-018-23874-6

[…] brilliant blue g250 and photographed with the powerlook 2100 xl scanner (umax). protein spots were detected and matched with the default parameters of the “spot detection wizard” function in the pdquest 8.0 program. the “find spot centers” function was used with default auto-noise smoothing and background subtraction. a gaussian model was applied to generate a master gel for each image file. […]


A Glycine Rich RNA Binding Protein, CsGR RBP3, Is Involved in Defense Responses Against Cold Stress in Harvested Cucumber (Cucumis sativus L.) Fruit

Front Plant Sci
PMCID: 5925850
PMID: 29740470
DOI: 10.3389/fpls.2018.00540

[…] bovine serum albumin (bsa) as the standard (). at least three biological replicates were used for each treatment and at least one gel was run for each replicate. after staining with coomassie blue, pdquest 2-de analysis software (version 8.0, bio-rad) was used to analyze the 2-de images (). automated detection and manual editing were carried out to obtain the highest gel matching. to account […]


Metabolic and Evolutionary Insights in the Transformation of Diphenylamine by a Pseudomonas putida Strain Unravelled by Genomic, Proteomic, and Transcription Analysis

Front Microbiol
PMCID: 5897751
PMID: 29681895
DOI: 10.3389/fmicb.2018.00676

[…] then by sds–page using 12.5% tris-hcl polyacrylamide gels (bio-rad) following standard procedures. 2-d gels were silver stained and scanned with bio-rad gs-800 calibrated densitometer equipped with pdquest advanced 2-d gel analysis software (version 8.1, bio-rad) as described by ziogas et al. (). data were analyzed by one-way anova (p ≤ 0.05) and means were compared using student's t-test […]


Proteomic analysis of antimicrobial effects of pegylated silver coated carbon nanotubes in Salmonella enterica serovar Typhimurium

PMCID: 5870919
PMID: 29587743
DOI: 10.1186/s12951-018-0355-0

[…] gels per bacterial culture conditions (0 or 25 µg/ml pswcnt-ag) were obtained for scanning using proteome works plus spot cutter (bio-rad). all images were aligned and protein spots were detected (pdquest 2-d analysis software v7, bio-rad). each spot was normalized based on the total valid spot intensity and protein quantitative analysis was performed by student’s t test at p < 0.05. […]


Blood coagulation abnormalities in multibacillary leprosy patients

PLoS Negl Trop Dis
PMCID: 5863944
PMID: 29565968
DOI: 10.1371/journal.pntd.0006214

[…] algorithm []., gels were stained with colloidal coomassie brilliant blue g-250 and documented using a gs-800 auto-calibrating imaging densitometer (bio-rad). image analysis was performed using pdquest software, version 8.0.1 (bio-rad). comparative 2d data were derived from three biological replicates from each clot type (control clot and leprosy clot). the spots were quantified based […]


Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down Regulating CDKL3

Front Physiol
PMCID: 5870041
PMID: 29615928
DOI: 10.3389/fphys.2018.00234

[…] protean ii xi cell system (bio-rad, california, usa). the proteins resolved in the gel were visualized by the modified silver stain, scanned with bio-rad versadoc imaging system, and analyzed using pdquest 8.0.1 software (bio-rad, california, usa). the selected protein spots were analyzed with abi 5800 plus maldi-tof/tof spectrometer (applied biosystems, california, usa) after in-gel digestion. […]

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