PDQuest 2-D protocols

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PDQuest 2-D specifications

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Unique identifier OMICS_02539
Name PDQuest 2-D
Alternative name PDQuest
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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PDQuest 2-D in pipelines

 (2)
2011
PMCID: 3031555
PMID: 21305017
DOI: 10.1371/journal.pone.0016334

[…] stage., 2-de proteomic maps of three hibm biopsies and their matched controls were generated using 20 cm gels over a ph range of 3.0–11.0. each hibm biopsy was compared to its matched control by pdquest 7.3.1 software () and only spots significantly differentially expressed (student's test; p<0.05) in all 3 hibm samples versus all 3 controls were identified by ms/ms spectra. peptides […]

2006
PMCID: 1488847
PMID: 16526958
DOI: 10.1186/1471-2164-7-46

[…] was performed on a home-made polyacrylamide gradient gel 8–16%. the gels were stained with see-band forte (geba). images were taken with a flours imager (biorad) and the images were analysed using pdquest software (biorad). spots were excised, in-gel digested with trypsin, and identified either by peptide mass fingerprinting and cid using a 4700 maldi-tof-tof mass spectrometer (applied […]


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PDQuest 2-D in publications

 (792)
PMCID: 5940786
PMID: 29739966
DOI: 10.1038/s41598-018-23874-6

[…] brilliant blue g250 and photographed with the powerlook 2100 xl scanner (umax). protein spots were detected and matched with the default parameters of the “spot detection wizard” function in the pdquest 8.0 program. the “find spot centers” function was used with default auto-noise smoothing and background subtraction. a gaussian model was applied to generate a master gel for each image file. […]

PMCID: 5925850
PMID: 29740470
DOI: 10.3389/fpls.2018.00540

[…] bovine serum albumin (bsa) as the standard (). at least three biological replicates were used for each treatment and at least one gel was run for each replicate. after staining with coomassie blue, pdquest 2-de analysis software (version 8.0, bio-rad) was used to analyze the 2-de images (). automated detection and manual editing were carried out to obtain the highest gel matching. to account […]

PMCID: 5897751
PMID: 29681895
DOI: 10.3389/fmicb.2018.00676

[…] then by sds–page using 12.5% tris-hcl polyacrylamide gels (bio-rad) following standard procedures. 2-d gels were silver stained and scanned with bio-rad gs-800 calibrated densitometer equipped with pdquest advanced 2-d gel analysis software (version 8.1, bio-rad) as described by ziogas et al. (). data were analyzed by one-way anova (p ≤ 0.05) and means were compared using student's t-test […]

PMCID: 5870919
PMID: 29587743
DOI: 10.1186/s12951-018-0355-0

[…] gels per bacterial culture conditions (0 or 25 µg/ml pswcnt-ag) were obtained for scanning using proteome works plus spot cutter (bio-rad). all images were aligned and protein spots were detected (pdquest 2-d analysis software v7, bio-rad). each spot was normalized based on the total valid spot intensity and protein quantitative analysis was performed by student’s t test at p < 0.05. […]

PMCID: 5863944
PMID: 29565968
DOI: 10.1371/journal.pntd.0006214

[…] algorithm []., gels were stained with colloidal coomassie brilliant blue g-250 and documented using a gs-800 auto-calibrating imaging densitometer (bio-rad). image analysis was performed using pdquest software, version 8.0.1 (bio-rad). comparative 2d data were derived from three biological replicates from each clot type (control clot and leprosy clot). the spots were quantified based […]


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