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pepStat specifications


Unique identifier OMICS_04027
Name pepStat
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


No version available


Publication for pepStat

pepStat citations


In silico functional elucidation of uncharacterized proteins of Chlamydia abortus strain LLG

PMCID: 5351547
PMID: 28344832
DOI: 10.4155/fsoa-2016-0066

[…] pathicity. Additionally, the properties of sequences including aromatic and aliphatic, basic and acidic sequences with average number of polar and nonpolar amino acids were determined by using EMBOOS PepStat []. To discover the position of these proteins with predicted signature sequences, TMHMM [], HMMTOP [] and SOSUI-GramN [] were used, while subcellular localization of proteins was determined b […]


Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in Hominoids

Sci Rep
PMCID: 5138847
PMID: 27922051
DOI: 10.1038/srep38302

[…] he atomic coordinates of DrUox. The number of cysteines in Uox proteins of different taxonomic divisions was determined for 1385 sequences clustered at 80% similarity with the cdhit program using the pepstat program of the Emboss package. Data were plotted using the geom_boxplot and geom_jitter functions of the ggplot2 library of the R package. […]


In Silico Phylogenetic Analysis and Molecular Modelling Study of 2 Haloalkanoic Acid Dehalogenase Enzymes from Bacterial and Fungal Origin

Adv Bioinformatics
PMCID: 4736600
PMID: 26880911
DOI: 10.1155/2016/8701201

[…] noic acid dehalogenase from bacterial and fungal sources were retrieved from the NCBI database ( The amino acid composition of these sequences was computed using PEPSTAT module integrated in the EMBOSS software []. Multiple sequence alignment for individual profiles was performed using MUSCLE and phylogenetic analysis using MEGA 6 software []. The discovered m […]


Protein Thermostability Is Owing to Their Preferences to Non Polar Smaller Volume Amino Acids, Variations in Residual Physico Chemical Properties and More Salt Bridges

PLoS One
PMCID: 4503463
PMID: 26177372
DOI: 10.1371/journal.pone.0131495

[…] mologous two hundred mesophilic proteins (Table A in ) were downloaded and the percentage of 20 amino acids in each protein was calculated by accessing the website []. The frequency zone was arranged in ascending order. Here, the Sturges formula (k = 1+3.322 log10 N) was used to find the class interval and desirable number of groups into which the distri […]


In silico characterization of putative drug targets in Staphylococcus saprophyticus, causing bovine mastitis

PMCID: 3669785
PMID: 23750077
DOI: 10.6026/97320630009339

[…] his is required to find out the surface membrane proteins which could be probable drug targets. Further these surface membrane pathway protein sequences were characterized by using Peptide Statistic (PEPSTAT) program which is available online via European Molecular Biology Open Software Suite (EMBOSS) web server []. […]


A top down approach to classify enzyme functional classes and sub classes using random forest

EURASIP J Bioinform Syst Biol
PMCID: 3351021
PMID: 22376768
DOI: 10.1186/1687-4153-2012-1

[…] ical information about an enzyme and if coupled with structural characteristics, can contribute to a more robust and accurately predicting model. By using 73 different features extracted using EMBOSS PEPSTAT and ProtParams tool, we have tried to highlight how existing tools can be re-used and extended to address interesting problems in Bioinformatics. The results from the experiments demonstrate t […]


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pepStat institution(s)
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

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