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Peptide assignment validation software tools | Mass spectrometry-based untargeted proteomics

Peptide assignment validation software tools | Mass spectrometry-based untargeted proteomics

In a typical proteomic analysis, the tandem mass spectra from a LC-MS/MS experiment are assigned to a peptide by a search engine that compares the experimental MS/MS peptide data to theoretical peptide sequences in a protein database. The peptide spectra matches are then used to infer a list of identified proteins in the original sample. However, the search engines often fail to distinguish between correct and incorrect peptides assignments.

Source text:
(Jian et al., 2013) A novel algorithm for validating peptide identification from a shotgun proteomics search engine. J Proteome Res.

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