PerlPrimer protocols

PerlPrimer specifications

Information


Unique identifier OMICS_02354
Name PerlPrimer
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

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Publication for PerlPrimer

PerlPrimer IN pipelines

 (3)
2017
PMCID: 5545845
PMID: 28784084
DOI: 10.1186/s12864-017-3972-3

[…] protein lorelei, anti pcd, and protein kinase-like protein), whereas the other four were downregulated (sui 1, polyubiquitin, pectate lyase and pectin esterase). primers were designed using the perlprimer software [44] to obtain melting temperatures (tm) ranging from 59 to 61 °c and fragment sizes from 100 bp to 250 bp. mpg and pt6 mrna was isolated as described above. per sample, 1 μg mrna […]

2015
PMCID: 5008137
PMID: 25907143
DOI: 10.1111/mec.13216

[…] to be specific to otus with identical classification and to include the two hypervariable regions v3 and v4 in the amplified fragment. the efficiency of the designed primers was then tested with perlprimer v.1.1.21 (marshall 2004)., for m. symmetochus guest ants and sericomyrmex host ants, we first generated sequencing data for 384 bp of mitochondrial cytochrome oxidase i (mtcoi) […]

2013
PMCID: 3589449
PMID: 23472125
DOI: 10.1371/journal.pone.0057973

[…] the masked sequences were analyzed with tandem repeat finder [23] to detect microsatellite repeats. two microsatellites were selected for each gene and primers were designed with perlprimer v1.1.13 (table s1)., samples came from a pedigree containing 17 cases with and 13 healthy dogs without a shunt (figure 1). a phage m13-based tag (gttttcccagtcacgac) was added at the 5′ end […]

PerlPrimer institution(s)
Murdoch Childrens Research Institute, Royal Childrens Hospital, Parkville, Vic, Australia

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