Provides eight vertebrate genomes for guide RNA (gRNA) design. pgRNAFinder contains three different modules: (1) a single gRNA design module, (2) a short distance paired-gRNA design module and (3) a long distance paired-gRNA design module. It incorporates other gRNA-designed tools to search and evaluate gRNA. This tool has a running time correlated with gRNA number, and off-target evaluation is the time-consuming step.
Key Laboratory of Gene Engineering of the Ministry of Education and State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China; School of Data and Computer Science, Sun Yat-Sen University, Guangzhou, China; Guangdong Province Key Laboratory of Big Data Analysis and Processing, Sun Yat-Sen University, Guangzhou, China; Cell-Based Assay Screening Core, Houston, TX, USA; Dan L. Duncan Cancer Center, Houston, TX, USA; Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA; Key Laboratory of Reproductive Medicine of Guangdong Province, Guangzhou, China
pgRNAFinder funding source(s)
Supported by National Natural Science Foundation of China (NSFC) (Grant 31171397, 31271533, and 31301085), Pearl River Nova Program of Guangzhou (2012T50739 and 201710010044) and National Natural Science Funds of Guangdong Province for Distinguished Young Scholar (2014A030306044).