PhotochemCAD specifications


Unique identifier OMICS_24374
Name PhotochemCAD
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Computer skills Medium
Version 2.1
Stability Stable
Maintained Yes



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  • person_outline Jonathan Lindsey <>
  • person_outline Masahiko Taniguchi <>

Additional information

Publications for PhotochemCAD

PhotochemCAD in publications

PMCID: 5407667
PMID: 28287526
DOI: 10.3791/54782

[…] to be measured. these measurements can be carried out in bulk, using a standard spectrometer and a standard fluorescence spectrometer. for each pair, the r0 is then calculated using the freeware photochemcad and can be used in the nps analysis. moreover, the (time-resolved) fluorescence anisotropies of the dye molecules need to be obtained using a polarization (and time) sensitive […]

PMCID: 5512671
PMID: 28054547
DOI: 10.1038/ncomms13972

[…] the yfp chromophore and acceptor pigments in the rc–lh1 complex, on the basis of the 0.48 value for φeet determined from the whole cell fluorescence lifetime measurements. the calculations employed photochemcad using three values of the refractive index (1.335, 1.45 and 1.55) to accommodate latitude in the polarity of the cofactor/protein environments (). the calculations return a yfp […]

PMCID: 5204206
PMID: 28058293
DOI: 10.1021/acsomega.6b00356

[…] donor fluorescence intensity, and εa(λ) is the absorbance spectrum of the acceptor normalized to its maximum molar extinction coefficient.theoretical förster distances were calculated using the photochemcad software, and the measured extinction coefficient for each probe were φtryptophan = 0.2, ηphosphate buffer, ph 7.4 = 1.33, and κ = 2/3., different concentrations of sa protein, chosen […]

PMCID: 4718142
PMID: 25926406
DOI: 10.1039/c5nr01402f

[…] μm solution of r6g in ethanol were dropped onto the surface of the cpg piece while the clsm was already scanning. r6g exhibits an absorption maximum at 530 nm and an emission maximum at 552 nm (cf. photochemcad package, version 2.1a). the scanned area extended 250 μm (481 pixel) in z direction perpendicular to the cpg membrane/glue/cover slide interface and 52 μm (256 pixel) in x-direction […]

PMCID: 3871636
PMID: 24376769
DOI: 10.1371/journal.pone.0083898

[…] as the estimates of τd., distances were calculated using the equation:(eq.3)where r0 is the förster distance, and r is the distance between the donor and acceptor. r0 was calculated with the use of photochemcad software . in these calculations, we used the values of the orientation factor (κ2) and the refractive index (n) of 0.667 and 1.4, respectively., to analyze the results of lret […]

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PhotochemCAD institution(s)
Department of Chemistry, North Carolina State University, Raleigh, NC, USA
PhotochemCAD funding source(s)
Supported chiefly by North Carolina State University, the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Chemical Sciences, Geosciences and Biosciences Division, under Award Number DE-FG02-96ER14632.

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