PhotochemCAD statistics

Tool stats & trends

Looking to identify usage trends or leading experts?


PhotochemCAD specifications


Unique identifier OMICS_24374
Name PhotochemCAD
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Computer skills Medium
Version 2.1
Stability Stable
Maintained Yes




No version available



  • person_outline Jonathan Lindsey
  • person_outline Masahiko Taniguchi

Additional information

Publications for PhotochemCAD

PhotochemCAD citations


Structural Information from Single molecule FRET Experiments Using the Fast Nano positioning System

PMCID: 5407667
PMID: 28287526
DOI: 10.3791/54782

[…] need to be measured. These measurements can be carried out in bulk, using a standard spectrometer and a standard fluorescence spectrometer. For each pair, the R0 is then calculated using the freeware PhotochemCAD and can be used in the NPS analysis. Moreover, the (time-resolved) fluorescence anisotropies of the dye molecules need to be obtained using a polarization (and time) sensitive fluorescenc […]


Augmenting light coverage for photosynthesis through YFP enhanced charge separation at the Rhodobacter sphaeroides reaction centre

Nat Commun
PMCID: 5512671
PMID: 28054547
DOI: 10.1038/ncomms13972

[…] n the YFP chromophore and acceptor pigments in the RC–LH1 complex, on the basis of the 0.48 value for ΦEET determined from the whole cell fluorescence lifetime measurements. The calculations employed PhotochemCad using three values of the refractive index (1.335, 1.45 and 1.55) to accommodate latitude in the polarity of the cofactor/protein environments (). The calculations return a YFP to accepto […]


Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore

PMCID: 5204206
PMID: 28058293
DOI: 10.1021/acsomega.6b00356

[…] lized donor fluorescence intensity, and εA(λ) is the absorbance spectrum of the acceptor normalized to its maximum molar extinction coefficient.Theoretical Förster distances were calculated using the PhotoChemCAD software, and the measured extinction coefficient for each probe were Φtryptophan = 0.2, ηphosphate buffer, pH 7.4 = 1.33, and κ = 2/3. […]


Adiabatic burst evaporation from bicontinuous nanoporous membranes

PMCID: 4718142
PMID: 25926406
DOI: 10.1039/c5nr01402f
call_split See protocol

[…] 0 μM solution of R6G in ethanol were dropped onto the surface of the CPG piece while the CLSM was already scanning. R6G exhibits an absorption maximum at 530 nm and an emission maximum at 552 nm (cf. PhotochemCAD package, version 2.1a). The scanned area extended 250 μm (481 pixel) in z direction perpendicular to the CPG membrane/glue/cover slide interface and 52 μm (256 Pixel) in x-direction paral […]


A Large Scale Allosteric Transition in Cytochrome P450 3A4 Revealed by Luminescence Resonance Energy Transfer (LRET)

PLoS One
PMCID: 3871636
PMID: 24376769
DOI: 10.1371/journal.pone.0083898

[…] 2∶1 as the estimates of τd.Distances were calculated using the equation:(Eq.3)where R0 is the Förster distance, and R is the distance between the donor and acceptor. R0 was calculated with the use of PhotoChemCad software . In these calculations, we used the values of the orientation factor (κ2) and the refractive index (n) of 0.667 and 1.4, respectively.To analyze the results of LRET titrations a […]


Walkable Dual Emissions

Sci Rep
PMCID: 3712315
PMID: 23857434
DOI: 10.1038/srep02199

[…] energy transfer from PAnPO2 to the lanthanide acceptors. And the Dexter energy transfer mechanism is operative in 1·Eu and 1·Tb based on the Calculations of donor/acceptor spectrosocpic overlap with PhotoChemCAD version 2.1. […]


Looking to check out a full list of citations?

PhotochemCAD institution(s)
Department of Chemistry, North Carolina State University, Raleigh, NC, USA
PhotochemCAD funding source(s)
Supported chiefly by North Carolina State University, the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Chemical Sciences, Geosciences and Biosciences Division, under Award Number DE-FG02-96ER14632.

PhotochemCAD reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review PhotochemCAD