pI calculator statistics

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pI calculator specifications


Unique identifier OMICS_19760
Name pI calculator
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input data A list of peptide sequences with their phosphorylated amino acids marked.
Operating system Unix/Linux
Programming languages Java
Computer skills Advanced
Stability No
Maintained No


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Publication for pI calculator

pI calculator in publications

PMCID: 5485175
PMID: 28249362
DOI: 10.1002/btpr.2455

[…] proteins identified were checked individually against the uniprot database using accession ids to determine biological functions (proteolytic activity). isoelectric point was calculated using expasy pi calculator online tool (expasy. compute pi/mw tool). hydropathicity index (gravy) was also calculated based on amino acidic sequence. relative percentage of each protein out of the total were also […]

PMCID: 4427354
PMID: 25961303
DOI: 10.1371/journal.pone.0126458

[…] 2 independent peptides., data obtained from the ief fractionation were further elaborated. theoretical values of pi peptides belonging to the same ief fraction were calculated in batch using the “pi calculator” software []. as explained elsewhere, we took advantage of the pi filtering application to optimize the cross correlation parameter (xcorr) to assess the performance of different […]

PMCID: 4103569
PMID: 23059722
DOI: 10.1264/jsme2.ME12091

[…] surface-associated protein are necessary to elucidate the physiological role of cell surface-associated protein., molecular mass and pi were calculated using expasy server http://www.expasy.ch/mw/pi calculator., signal peptides were predicted with our sequence results () and the information based on cyanobase (http://genome.kazusa.or.jp/cyanobase/anabaena) and signalp 4.0 […]

PMCID: 3442994
PMID: 22672774
DOI: 10.1186/1477-5956-10-37

[…] sequence-determined properties, may even have a shift to a more acidic pi. in this study, a first effort was done to describe these properties. it is noteworthy to point out that to date, most pi calculator algorithms use only native peptide sequences without taking into account the itraq tags. consequently, further experiments in combination with trustworthier, advanced pi calculator […]

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pI calculator institution(s)
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands; Hubrecht Institute, Utrecht, Netherlands
pI calculator funding source(s)
Supported by the Netherlands Proteomics Centre and the Netherlands Bioinformatic Centre.

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