Parallel iterative deconvolution

[…] software (molecular devices). images were taken as z-stacks in a range of 10–14 µm, with a distance between planes of 0.2 μm., deconvolution in three spatial dimensions was performed with the parallel iterative deconvolution plugin of imagej, using the wiener filter preconditioned landweber algorithm with the following parameters: weiner = 0.001 (expressed as a fraction of the largest […]

[…] all image processing and analyses was performed in fiji. to process images for quantification of synaptic profiles, first z-stacks were deconvolved using the wpl deconvolution algorithm in the parallel iterative deconvolution imagej plugin [] based on the iterative deconvolve 3d algorithm [] utilizing a theoretical 3d point spread function generated in psf lab []. image stacks […]

[…] undertaken using a rabbit polyclonal anti-psmad1/5/8 primary antibody (cell signaling 9511l, 1:150) and a secondary antibody (1:500) coupled to fitc. photographs were processed in imagej using the parallel iterative deconvolution 2d plug-in. xenopus embryos or animal caps were processed for in situ hybridization as described in . for quantitative rt-pcr, total rnas were extracted […]

[…] to further recover the image from blurring and substantially enhance the resolution. the iterative 3-d deconvolution conducted in this work was run on the open source imagej platform using its “parallel iterative deconvolution” plugin, “3-d iterative deconvolution” module. we selected the mrnsd (modified residual norm steepest descent) or wpl (wiener filter preconditioned landweber) option, […]

[…] alexa 647 donkey anti-mouse (a-31571; life technologies) as secondary antibody. images were acquired with a nikon eclipse ti microscope. image stacks were deconvolved using nih imagej software with parallel iterative deconvolution plugin, and reconstituted maximum-intensity projections images are shown in the results., presence of ldh in cell-free supernatants was used as marker for cell death […]