pISTil specifications


Unique identifier OMICS_12801
Name pISTil
Alternative name pipeline for yeast two-hybrid Interaction Sequence Tags identification and analysis
Software type Pipeline/Workflow
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS
Programming languages Perl, PHP
Database management system PostgreSQL
License GNU General Public License version 3.0
Computer skills Medium
Stability Stable
Phred, Apache, Staden, NCBI BLAST Toolkit
Maintained Yes


No version available



  • person_outline Johann Pellet

Publication for pipeline for yeast two-hybrid Interaction Sequence Tags identification and analysis

pISTil citation


The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication

PLoS Pathog
PMCID: 3701712
PMID: 23853584
DOI: 10.1371/journal.ppat.1003440

[…] one time the libraries. Positive clones were maintained onto this selective medium for 15 days to eliminate any contaminant AD-cDNA plasmid. AD-cDNAs were PCR-amplified, sequenced and analyzed using pISTil . Cellular ORFs (interacting domains found in Y2H screens) were amplified from a pool of human cDNA libraries or from a plasmid encoding the corresponding cDNA from the MGC collection (IMAGE co […]

pISTil institution(s)
U851, INSERM, Lyon, France; Université de Lyon, Lyon, France; Université Lyon 1, Lyon, France; Laboratoire de Génomique Virale et Vaccination, Institut Pasteur, CNRS, URA 3015, Paris, France; 5UMR754, INRA, ENVL, Lyon, France; Centre de RMN à Très Hauts Champs, CNRS, France
pISTil funding source(s)
This work was funded by INSERM, Université Lyon 1, INRA, the Association de la Recherche contre le Cancer, the Ligue Nationale Contre le Cancer and the French Ministry of Industry, and supported by a grant from INRA.

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