PITCh designer specifications


Unique identifier OMICS_18464
Name PITCh designer
Alternative name Precise Integration into Target Chromosome designer
Interface Web user interface
Restrictions to use None
Input data A nucleotide sequence (100-15,000 bp).
Input format FASTA
Output data A list of potential knock-in sites within the provided sequence.
Programming languages Javascript
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Tetsushi Sakuma <>

Publication for Precise Integration into Target Chromosome designer


Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO.

2017 Bioeng Bugs
PMID: 28453368
DOI: 10.1080/21655979.2017.1313645
PITCh designer institution(s)
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan
PITCh designer funding source(s)
Supported in part by a Grant-in-Aid for Young Scientists (B) (16K18478) and a Grant-in-Aid for JSPS Research Fellows (16J03164).

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