PixFRET specifications

Information


Unique identifier OMICS_25530
Name PixFRET
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Computer skills Medium
Stability Stable
Maintained No

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Publication for PixFRET

PixFRET in publications

 (27)
PMCID: 5821396
PMID: 29466456
DOI: 10.1371/journal.pone.0193279

[…] and venus only (hmp-1::venus(cter)), respectively., all images were analysed using imagej (wayne rasband, national institute of health, usa). sbt and fret index were computed using the plugin pixfret []. the dbt and abt were measured the same day or the day after fret measurements. their values were derived using the following equations: dbt=ifretidonor(usinghmp‑1::mtfp1(cter)strain)(1) […]

PMCID: 5803212
PMID: 29416033
DOI: 10.1038/s41467-018-02929-2

[…] during confocal imaging all settings were kept constant (for details, see methods). to evaluate the occurrence of fret, we analyzed the images using a method that was previously reported, where the pixfret plugin of the image processing software imagej was used for fret quantification. briefly, the bleed-through of the acceptor and donor channels was determined and finally the calculated fret […]

PMCID: 5498622
PMID: 28680155
DOI: 10.1038/s41598-017-04914-z

[…] were determined by using cells transfected with yfp or cfp alone. we used the following equation: e = fretc /(cfp + fretc) × 100% to quantify the fret signal by using metafluo and image j software (pixfret plug-in), –., single-cell migration assay was described previously, , , . in brief, the purified oecs were re-plated onto square coverslips (8 mm) coated with laminin (10 μg/ml) at a low […]

PMCID: 5492851
PMID: 28713242
DOI: 10.3389/fnmol.2017.00210

[…] same process of a in the imagej plugin. typical values in our experimental conditions are a = 0.1 and b = 0.25, respectively. normalized fret (nfret) was performed with the imagej software plugin “pixfret” (feige et al., ) by using: nfret = findex/√(id × ia) (xia and liu, ). mathematically, nfret values should range between 0–1; the plugin automatically multiplies these values ×100; nfret […]

PMCID: 5540479
PMID: 28661401
DOI: 10.7554/eLife.25233.032

[…] dividing the raw image with a background image using imagej version 1.51 f (). correction for donor bleedthrough (6%) and generation of nfret images (tr-fret/(donor × acceptor)0.5) was done with the pixfret plugin to imagej (). acceptor bleedthrough and direct acceptor excitation was not detected. for quantification, all non-zero pixels in the nfret image were selected, pixels not belonging […]


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PixFRET institution(s)
Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Switzerland; Biomedical Imaging Group, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland

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