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pLabel specifications

Information


Unique identifier OMICS_02469
Name pLabel
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Medium
Version 2.4.0.8
Stability Stable
Maintained Yes

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pLabel citations

 (18)
library_books

Mapping disulfide bonds from sub micrograms of purified proteins or micrograms of complex protein mixtures

2018
Biophysics Rep
PMCID: 5937861
PMID: 29756007
DOI: 10.1007/s41048-018-0050-6

[…] first time. to receive the license file, send an e-mail to [email protected] follow instructions during installation. the plink 2 installation package includes pparse, a ms data conversion tool, and plabel—a very convenient and powerful tool for annotating peaks in a ms2 spectrum. once plink 2 is installed, pparse and plabel are ready.python 3.6: installation instructions are available […]

library_books

Polη O GlcNAcylation governs genome integrity during translesion DNA synthesis

2017
Nat Commun
PMCID: 5717138
PMID: 29208956
DOI: 10.1038/s41467-017-02164-1

[…] as differential modification. search results were filtered using dtaselect 2.0 with a 5% false discovery rate cutoff at the spectral level. the glcnac-modified peptide spectrum was labeled using plabel, requiring 20 ppm mass accuracy for fragment ions., cells were lysed in csk-100 buffer (100 mm nacl, 300 mm sucrose, 3 mm mgcl2, 10 mm pipes ph 6.8, 1 mm egta, 0.2% triton x-100) containing […]

library_books

O GlcNAcylation of SIRT1 enhances its deacetylase activity and promotes cytoprotection under stress

2017
Nat Commun
PMCID: 5684413
PMID: 29133780
DOI: 10.1038/s41467-017-01654-6

[…] xl™ etd mass spectrometer analysis, the ion source voltage is set to 1500 v. ms/ms data were searched using the pfind program for o-glcnacylated peptides, and the spectra were annotated using plabel–., the collected purified proteins (300 ng) were incubated with gst fusion proteins (100–200 ng) bound on gs4b beads (ge healthcare) overnight at 4 °c. the beads were then washed three times […]

library_books

In conditions of over expression, WblI, a WhiB like transcriptional regulator, has a positive impact on the weak antibiotic production of Streptomyces lividans TK24

2017
PLoS One
PMCID: 5373594
PMID: 28358920
DOI: 10.1371/journal.pone.0174781

[…] genomic dna by pcr using primer pairs bs3579f/bs3579r, bs5046f/p5046r, bs5078f/bs5078r, bs82-83f/bs82-83r (), respectively. the resulting pcr products were 5’ labeled with fitc by pcr using primer plabel (). 50 pmol of each of the promoter regions was incubated with purified his6-wbli in various concentrations for 15 min at room temperature in binding buffer (10 mm tris-hcl, 50 mm kcl, 1mm […]

library_books

Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis

2017
PMCID: 5364692
PMID: 28344766
DOI: 10.1186/s13578-017-0142-x

[…] []. search results were filtered using dtaselect 2.0 [] with 7 p.p.m. mass accuracy for precursor mass and a 5% fdr cutoff. the phosphorylation spectra presented in the figures were annotated using plabel []., chromatin fractionation was performed essentially as described before []. hela and hek293 cells were synchronized at metaphase by thymidine-nocodazole block as described before []., bin […]

library_books

A panel of four genes accurately differentiates benign from malignant thyroid nodules

2016
PMCID: 5084448
PMID: 27793213
DOI: 10.1186/s13046-016-0447-3

[…] \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \mathrm{plabel}=\mathrm{s}\mathrm{g}\mathrm{n}\left(\sigma \mathrm{n}\mathrm{i}=0\ \mathrm{w}\mathrm{i}\ \exp \left(\hbox{-} \mathrm{gamma}\left|\ \left(\mathrm{xi}\hbox{-} […]


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