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pLabel specifications


Unique identifier OMICS_02469
Name pLabel
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Maintained Yes




No version available


pLabel citations


Mapping disulfide bonds from sub micrograms of purified proteins or micrograms of complex protein mixtures

Biophysics Rep
PMCID: 5937861
PMID: 29756007
DOI: 10.1007/s41048-018-0050-6

[…] e pLink 2 installation package includes pParse, a MS data conversion tool, and pLabel—a very convenient and powerful tool for annotating peaks in a MS2 spectrum. Once pLink 2 is installed, pParse and pLabel are ready.Python 3.6: Installation instructions are available at package “openpyxl” and “xlrd”: Enter in the following commands in windows “Command Prompt”:pip3 i […]


Polη O GlcNAcylation governs genome integrity during translesion DNA synthesis

Nat Commun
PMCID: 5717138
PMID: 29208956
DOI: 10.1038/s41467-017-02164-1
call_split See protocol

[…] sine as differential modification. Search results were filtered using DTASelect 2.0 with a 5% false discovery rate cutoff at the spectral level. The GlcNAc-modified peptide spectrum was labeled using pLabel, requiring 20 ppm mass accuracy for fragment ions. […]


O GlcNAcylation of SIRT1 enhances its deacetylase activity and promotes cytoprotection under stress

Nat Commun
PMCID: 5684413
PMID: 29133780
DOI: 10.1038/s41467-017-01654-6
call_split See protocol

[…] rbitrap XL™ ETD mass spectrometer analysis, the ion source voltage is set to 1500 V. MS/MS data were searched using the pFind program for O-GlcNAcylated peptides, and the spectra were annotated using pLabel–. […]


Delineation of B cell Epitopes of Salmonella enterica serovar Typhi Hemolysin E: Potential antibody therapeutic target

Sci Rep
PMCID: 5438399
PMID: 28526816
DOI: 10.1038/s41598-017-01987-8
call_split See protocol

[…] e amino acids and one stop codon. Each synthesized oligonucleotide was assembled into double stranded DNA by a filling in reaction using Klenow fragment (NEB). The degenerate sequence was cloned into pLABEL phagemid vector with NcoI and NotI restriction sites and introduced into E. coli XL1-Blue competent cells (Agilent Technologies, CA) through electroporation. Approximately 400 transformations w […]


In conditions of over expression, WblI, a WhiB like transcriptional regulator, has a positive impact on the weak antibiotic production of Streptomyces lividans TK24

PLoS One
PMCID: 5373594
PMID: 28358920
DOI: 10.1371/journal.pone.0174781

[…] 24 genomic DNA by PCR using primer pairs BS3579F/BS3579R, BS5046F/P5046R, BS5078F/BS5078R, BS82-83F/BS82-83R (), respectively. The resulting PCR products were 5’ labeled with FITC by PCR using primer Plabel (). 50 pmol of each of the promoter regions was incubated with purified His6-WblI in various concentrations for 15 min at room temperature in binding buffer (10 mM Tris-HCl, 50 mM KCl, 1mM DTT, […]


Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis

PMCID: 5364692
PMID: 28344766
DOI: 10.1186/s13578-017-0142-x
call_split See protocol

[…] d []. Search results were filtered using DTASelect 2.0 [] with 7 p.p.m. mass accuracy for precursor mass and a 5% FDR cutoff. The phosphorylation spectra presented in the figures were annotated using pLabel []. […]


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