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plusTipTracker specifications

Information


Unique identifier OMICS_06579
Name plusTipTracker
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages MATLAB
Computer skills Medium
Stability Stable
Maintained Yes

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Publication for plusTipTracker

plusTipTracker citations

 (37)
library_books

Cytoskeleton stability is essential for the integrity of the cerebellum and its motor and affective related behaviors

2018
PMCID: 5814431
PMID: 29449678
DOI: 10.1038/s41598-018-21470-2

[…] three seconds for 5 minutes. all images were captured with a charge-coupled device camera (coolsnap hq; roper scientific, sarasota, florida, usa)., for the analysis of the microtubule dynamics the plustiptracker software and the gfp-eb3 video time-lapse images were employed. we first adjusted the accuracy of the gfp-eb3 detection, and then analyzed the following parameters: growing […]

library_books

EB1 phosphorylation mediates the functions of ASK1 in pancreatic cancer development

2017
PMCID: 5716725
PMID: 29228685
DOI: 10.18632/oncotarget.21004

[…] at 37°c in at atmosphere containing 5% co2. time-lapse images of gfp-eb1 comets were captured at 2-sec intervals for 2 min as described previously []. microtubule dynamics were analyzed using plustiptracker software [, ]., student's t-tests were performed for significant differences. chi-square (x2) tests were performed to determine the correlation between two samples., conflicts […]

library_books

Adenomatous polyposis coli nucleates actin assembly to drive cell migration and microtubule induced focal adhesion turnover

2017
PMCID: 5584174
PMID: 28663347
DOI: 10.1083/jcb.201702007

[…] 10 ms of exposure at 488-nm excitation and 10% laser power. images were captured at 1-s intervals for 5 min, maintaining cells at 37°c using a heated stage (ibidi). gfp-eb1 comets were tracked using plustiptracker utrack version 2.1.3 software (), and comet velocities, lifetimes, and dynamicity were calculated in matlab (version r2015a)., to test the effects of apc silencing and rescue on fa […]

library_books

CLASP1 regulates endothelial cell branching morphology and directed migration

2017
PMCID: 5665473
PMID: 28860131
DOI: 10.1242/bio.028571

[…] sirna (control)., to assess the influence of clasp1-mediated regulation of mt dynamics, we measured mt growth speed and growth lifetime in cells with altered clasp1 expression levels using plustiptracker automated image analysis of time-lapse images of mapple-eb3 (; ; ). comparison of mt growth parameters revealed that clasp1 sirna resulted in faster mt growth speeds, whereas […]

library_books

MCAK mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin II contractility

2017
PMCID: 5415018
PMID: 28298485
DOI: 10.1091/mbc.E16-05-0306

[…] pa ecms ( and supplemental figure s1). mt dynamics were visualized by tracking mapple eb3, a marker of mt plus-end growth, and mt growth speeds and mt growth lifetimes were analyzed using plustiptracker software (; ). mt plustiptracker software detects and tracks fluorescently labeled mt plus ends to calculate mt growth speeds and lifetimes (). plustiptracker also generates image […]

library_books

The microtubule plus end tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development

2017
PMCID: 5312526
PMID: 28202041
DOI: 10.1186/s13064-017-0080-7

[…] inverted motorized microscope with a zeiss 63× plan apo 1.4 na and a hamamatsu orca r2 ccd camera. mt dynamics were assessed, as described []., mt dynamics were analyzed from gfp-macf43 movies using plustiptracker [, ]. imaging conditions and tracking parameters were previously validated and same parameters were used: maximum gap length is 8 frames; minimum track length is 3 frames; search […]


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plusTipTracker institution(s)
The Scripps Research Institute, La Jolla, CA, USA

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