Protocols

PoissonNMF specifications

Information


Unique identifier OMICS_25925
Name PoissonNMF
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Requirements
ImageJ
Source code URL https://owncloud.tuebingen.mpg.de/index.php/s/d7d468a74a4da00164b1d4be82fb1e21
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline Andre Zeug

Additional information


https://neherlab.wordpress.com/poissonnmf-documentation/

Publication for PoissonNMF

PoissonNMF citations

 (2)
library_books

Using spectral decomposition of the signals from laurdan derived probes to evaluate the physical state of membranes in live cells

2017
F1000Res
PMCID: 5473435
PMID: 28663788
DOI: 10.5256/f1000research.12507.r23165

[…] ery well suited for staining of live cells with C-laurdan. After attempting to use their “GP plugin”, we concur with their conclusions, and find that our approach based on spectral decomposition with PoissonNMF provides much clearer pictures, as well as being much less demanding in terms of calculating computer time.One important point to bear in mind, however, is that, although the results we rep […]

call_split

Next generation in vivo optical imaging with short wave infrared quantum dots

2017
PMCID: 5673283
PMID: 29119058
DOI: 10.1038/s41551-017-0056
call_split See protocol

[…] s) of the brain during injection of PEGylated QDs, three days after a similar injection to pre-label the tumor. Using this video we can perform a form of principal component analysis (PCA), using the PoissonNMF ImageJ plugin. This method identifies the extent to which each pixel can be represented as an artery or vein by relating its temporal profile to those of vessels of known identity. Any sign […]

PoissonNMF institution(s)
Kavli Institute for Theoretical Physics, University of California, Santa Barbara, CA, USA; Department of Molecular Biology of Neuronal Signals, Gottingen, Germany; Glial Physiology and Imaging, Neurogenetics, Max Planck Institute of Experimental Medicine Gottingen, Gottingen, Germany; Department Membranbiophysik, Max Planck Institute for Biophysical Chemistry Gottingen, Gottingen, Germany; Institute for Bioinformatics and Systems Biology, Helmholtz Zentrum Munchen, Neuherberg, Germany; Max Planck Institute for Dynamics and Self-Organization, Gottingen, Germany; Department of Neurophysiology and Cellular Biophysics, University of Gottingen, Gottingen, Germany; Deutsche Forschungsgemeinschaft-Research Center for the Molecular Physiology of the Brain, Gottingen, Germany
PoissonNMF funding source(s)
Supported by the Deutsche Forschungsgemeinschaft through the Research Center Molecular Physiology of the Brain (FZT 103 and EXC 171), by the National Science Foundation under grant No. NSF PHY05-51164, and by the Helmholtz Alliance on Systems Biology (project CoReNe).

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