PolyCat protocols

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PolyCat specifications


Unique identifier OMICS_07404
Name PolyCat
Software type Pipeline/Workflow
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C++, Perl
Computer skills Advanced
Stability Stable
SAMtools, BAMtools, BioPerl, Bio::DB::SAM
Maintained Yes


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PolyCat in publications

PMCID: 5777989
PMID: 29358676
DOI: 10.1038/s41598-018-19684-5

[…] heterozygosity greatly increases computational effort, hampering quantitative results per genome. consequently the rna reads are not separated and traced back to their (sub-) genome. algorithms like polycat and hands2 process reads based on classification towards their genome of origin, but heavily depend on the presence and the quality of reference genomes,. since not all cultivars of interest […]

PMCID: 5870093
PMID: 29373286
DOI: 10.1016/j.molmet.2018.01.003

[…] glargine treatment twice weekly according to blood glucose levels (accu-chek aviva, roche, mannheim, germany). glycated hemoglobin (hba1c) was determined by cation-exchange chromatography on a polycat a column . mice were kept in standard 12 h light/dark cycle and provided free access to standard chow diet and water. markers for retinopathy, neurodegeneration, and methylglyoxal measurement […]

PMCID: 5726920
PMID: 29131144
DOI: 10.1038/nchembio.2515

[…] 1100 (degasser, pumps, column compartment, uv module) and 1200 (fraction collector, fraction chiller) high-performance liquid chromatography setup. the catwax columns used contained equal amounts of polycat a and polywax lp, however two variations of columns were used: (1) 12 μm particle and 1500 å pore and (2) 5 μm particle and 1000 å pore. samples were run on exploratory and optimized […]

PMCID: 5681684
PMID: 29127281
DOI: 10.1038/s41598-017-13667-8

[…] signals as indications of the amount of s132p synthesized., analysis of different hemoglobin variants was performed by hplc using agilent 1100 series hplc system (agilent technologies, ca) and polycat atm weak cation-exchange column (100 × 4.6-mm, 3 μm, 1500 a; catalog #104ct0315, polylc inc., columbia, md) as previously described. the chromatographic separation was achieved at 24 °c […]

PMCID: 5886110
PMID: 28973399
DOI: 10.1093/hmg/ddx351

[…] (waters, milford, ma, usa) according to (). in short, the protocol consisted of a 30-min elution over a combined 20–200 mm nacl and ph 7.0–6.6 gradient in 20 mm bistris/hcl, 2 mm kcn. the column, a polycat a 100/4.6-mm, 3 μm, 1500 å column, was purchased from polylc (columbia, md, usa)., is available at hmg online., we thank the optical imaging centre (oic) of the erasmus mc for microscopy […]

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PolyCat institution(s)
Plant and Wildlife Science Department, Brigham Young University, Provo, Utah, USA

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