Pool sequencing data analysis bioinformatics software tools

Whole-genome sequencing of pools of individuals (Pool-seq) provides a cost-effective alternative to sequencing individuals separately. With the availability of custom-tailored software tools, Pool-seq is being increasingly used for population genomic research on both model and…

Pool-seq analysis steps

Most recent bioinformatics tools

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AXE
Desktop

AXE

Allows demultiplexing 5’-indexed reads. Axe matches the prefix of a sequence…

Allows demultiplexing 5’-indexed reads. Axe matches the prefix of a sequence read against a pre-computed trie of index sequences. It starts by calculating all sequences within a given hamming…

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PDA
Desktop

PDA Pooled DNA Analyzer

Permits to analyze pooled DNA data. PDA is based on a four-stage procedure that…

Permits to analyze pooled DNA data. PDA is based on a four-stage procedure that combines the concept of a three-stage DNA pooling experiment with a procedure of multipoint association test. It…

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EPDS
Desktop

EPDS

Identifies single nucleotide polymorphisms (SNPs) from pooled DNA samples. For…

Identifies single nucleotide polymorphisms (SNPs) from pooled DNA samples. For a program profile of wild given and pooled samples, EPDS can identify the mutant and estimates its proportion. This…

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ClonoCalc
Desktop

ClonoCalc

Prepares the raw data for downstream analyses. ClonoCalc combines a…

Prepares the raw data for downstream analyses. ClonoCalc combines a demultiplexer for barcode splitting and employs MiXCR for paired-end read merging and the extraction of human and animal T cell…

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