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Traditionally, the majority of flow cytometry experiments have been analyzed visually, either by serial manual inspection of one or two dimensions at a time (a process termed “gating”, with boundaries or “gates” defining cell populations of interest). However, these visual approaches are labor intensive and highly subjective, and they neglect information present in the data that are not visible to the human eye, thus representing a major obstacle to the automation and reproducibility of research.
(Finak., 2016) Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium. Scientific Reports.