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Associated diseases

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PriFi specifications


Unique identifier OMICS_26660
Name PriFi
Interface Web user interface
Restrictions to use None
Input data A multiple sequence alignment.
Input format FASTA,Clustal
Computer skills Basic
Stability Stable
Maintained Yes



  • person_outline Jakob Fredslund <>

Publication for PriFi

PriFi in publications

PMCID: 5861154
PMID: 29593778
DOI: 10.3389/fgene.2018.00075

[…] gene that is reported to be affected by glyphosate-based herbicides and salt treatments (shiogiri et al., ; armesto et al., )., the gene fragments were amplified by pcr using primers designed in the prifi online tool ( after alignments (supplementary table ) of known sequences deposited in the genbank (table ). the pcr was performed using the mix buffer gotaq® […]

PMCID: 5015932
PMID: 27371951
DOI: 10.1534/g3.116.029595

[…] codominant polymerase chain reaction-restriction length polymorphism (pcr-rflp) markers were used to determine genotypes for all progeny. markers were either taken from , , or , or designed using prifi () (supplemental material, table s1)., differences in trait values between jec20 and cdc15 were evaluated with the two-tailed student’s t–test. normality of traits of the progeny were evaluated […]

PMCID: 5008908
PMID: 27504806
DOI: 10.7554/eLife.18073.012

[…] just rewarded action a (note that these neurons have a higher firing rates than the other population) make transitions as following.(8)f1a+→f1a++∑i=1mαrifia−−pr1f1a+(9)f1<i<ma+→f1<i<ma++prifi−1a+−pri+1fia+(10)fma+→fma+−prifm−1a+(11)f1<i<ma−→f1<i<ma−−αrif1<i<ma−, where αri is the transition probability to modify synaptic strength (between depressed 0 and 1) […]

PMCID: 3703533
PMID: 23847608
DOI: 10.3389/fmicb.2013.00185

[…] sp. sb155-2, sulfurovum sp. nbc-37, and sulfurimonas denitrificans dsm 1225 (table ). initial primer sequences were created using the soxb nucleotide sequences in primaclade (gadberry et al., ) and prifi (fredslund et al., ), and the amino acid sequences in icodehop (boyce et al., ). sequences were then manually adjusted based on the initial bacterial sequences. codehops are hybrid primers […]

PMCID: 3698922
PMID: 23682154
DOI: 10.1093/gbe/evt076

[…] results from the same species of the query., but5 and p-element primers bl, br, p3, and p13 were designed based on sequences obtained by bioinformatic searches (from d. buzzatii and d. mojavensis). prifi () was used to find the best regions to place the primers on multiple alignments. the rest of the primers were designed based on d. buzzatii p element (bu-73 strain). all primers were designed […]

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PriFi institution(s)
Bioinformatics Research Center, University of Aarhus, Aarhus, Denmark; Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Aarhus, Denmark
PriFi funding source(s)
Supported by the Danish Agricultural and Veterinary Research Council.

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