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Primer Express specifications

Information


Unique identifier OMICS_33327
Name Primer Express
Software type Application/Script
Interface Command line interface, Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
Computer skills Advanced
Version 3.0.1
Stability Stable
Maintained Yes

Versioning


No version available

Documentation


Publication for Primer Express

Primer Express citations

 (4831)
library_books

Risk factors associated with HTLV 1 vertical transmission in Brazil: longer breastfeeding, higher maternal proviral load and previous HTLV 1 infected offspring

2018
Sci Rep
PMCID: 5958084
PMID: 29773807
DOI: 10.1038/s41598-018-25939-y

[…] GGGTTTT-3′, HTLV-1 nucleotide 4943–4920). The internal HTLV-1 TaqMan probe (5′-CTTTACTGACAAACCCGACCTACCCATGGA-3′) was selected using the Oligo (version 4, National Biosciences, Plymouth, MI, USA) and Primer Express (Perkin-Elmer Applied Biosystems, Boston, MA, USA) software programs and checked through a search of GenBank. The probe was located between positions 4829 and 4858 of the HTLV-1 genome […]

call_split

Transcriptome Analysis of Circulating PBMCs to Understand Mechanism of High Altitude Adaptation in Native Cattle of Ladakh Region

2018
Sci Rep
PMCID: 5955995
PMID: 29769561
DOI: 10.1038/s41598-018-25736-7
call_split See protocol

[…] t functional categories viz., GAPDH, RPL4, EEF1A1, RPS9, HPRT, UXT, HMBS, B2M, RPS15 and ACTB. The primers for each of the ICGs and target genes were either selected from literature or designed using Primer express 3.0 software (Applied Biosystem). Primer details for all the ICGs and target genes are provided as supplementary information (Tables  and ). The accuracy of primer pairs was also ensure […]

library_books

Pheromone gland development and monoterpenoid synthesis specific to oviparous females in the pea aphid

2018
PMCID: 5946545
PMID: 29780614
DOI: 10.1186/s40851-018-0092-0

[…] ribed with the High Capacity cDNA Reverse Transcription Kit based on the manufacturer’s instructions (Applied Biosystems, Foster City, CA, USA).Primers for the target enzyme genes were designed using Primer Express software v3.0.1 (Applied Biosystems) (Additional file : Table S1). For AACT1 and AACT2, the primers were designed to cover the conserved region shared by the two paralogs, because the D […]

call_split

Sika deer (Cervus nippon) specific real time PCR method to detect fraudulent labelling of meat and meat products

2018
Sci Rep
PMCID: 5940659
PMID: 29739996
DOI: 10.1038/s41598-018-25299-7
call_split See protocol

[…] arity was detected via online BLAST (Basic Local Alignment Algorithm Search Tool), sequence alignment was performed with the CLC Genomics Workbench 8.0 (Qiagen). Primers and probes were designed with Primer Express 3.0 (Applied Biosystems). Probes were labelled with DFO (Dragonfly Orange) at the 5′ end and a non-fluorescent minor groove binding quencher (MGBNFQ) at the 3′ end. Primers were synthes […]

library_books

Neuropilin 1 promotes the oncogenic Tenascin C/integrin β3 pathway and modulates chemoresistance in breast cancer cells

2018
BMC Cancer
PMCID: 5935908
PMID: 29728077
DOI: 10.1186/s12885-018-4446-y

[…] RNA extraction and qRT-PCR were performed according to standard protocols as described earlier []. Primers were designed using the Primer Express software (Applied Biosystems, USA) and are listed in Additional file : Table S2. […]

library_books

The Expression of AQP1 IS Modified in Lung of Patients With Idiopathic Pulmonary Fibrosis: Addressing a Possible New Target

2018
Front Mol Biosci
PMCID: 5943501
PMID: 29774214
DOI: 10.3389/fmolb.2018.00043

[…] and the thermocycler conditions recommended by the manufacturer. The expression of HPRT1 was used to normalize for differences in amounts of input DNA between samples. Primers were designed using the Primer Express software (version 2.0, Applied Biosystems, Warrington, UK) and their sequences are indicated in Table . Melting curve analysis showed a single sharp peak with the expected melting tempe […]

Citations

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Primer Express institution(s)
Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India
Primer Express funding source(s)
Supported by grants from University of Delhi, Department of Biotechnology (DBT), Department of Science and Technology (DST), and Council of Scientific and Industrial Research (CSIR), India.

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