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Primer Premier specifications


Unique identifier OMICS_29404
Name Primer Premier
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Mac OS, Windows
License Commercial
Computer skills Medium
Version 6.24
Stability Stable
Free trial Yes
Maintained Yes




No version available


  • person_outline Beacon Designer Team

Publication for Primer Premier

Primer Premier citations


Identification and tissue distribution of chemosensory protein and odorant binding protein genes in Tropidothorax elegans Distant (Hemiptera: Lygaeidae)

Sci Rep
PMCID: 5958050
PMID: 29773848
DOI: 10.1038/s41598-018-26137-6

[…] TaKaRa, Dalian, China). The T. elegans actin gene (Genbank accession no. MG322127) was used as control. Each reaction was performed with 200 ng/μl cDNA sample. Primers for RT-qPCR were designed using Primer Premier 5.0 software and are listed in Table . The RT-qPCR reactions were conducted in 20 μL reaction mixtures containing 10 μL SYBR Premix Ex Taq II, 20 ng cDNA templates, 0.2 μM of each prime […]


Different clusters of Candidatus ‘Methanoperedens nitroreducens’ like archaea as revealed by high throughput sequencing with new primers

Sci Rep
PMCID: 5955947
PMID: 29769540
DOI: 10.1038/s41598-018-24974-z

[…] alignment of these sequences was performed with Clustal X (version 2.0) and the conserved region was selected as the target for the primer design (Fig. ). The primer design was then carried out using Primer Premier (version 6.0, Premier Biosoft International, USA). The McrA 997 F primer was newly designed in this study and the efficiency was confirmed in silico with the NCBI Primer-Blast tool.Figu […]


Characterization of new microsatellite markers based on the transcriptome sequencing of Clematis finetiana

PMCID: 5952850
PMID: 29785177
DOI: 10.1186/s41065-018-0060-x

[…] r the qRT-PCR experiment. And 12 genes with different expression levels were randomly selected for validating the expression results of RNA-seq sequencing. Primers (Additional file ) were designed by Primer Premier 5( based on the selected unigene sequences. PCR reaction mixture was consisted of 7 μl ddH2O, 1 […]


Transcriptomic and metabolomic analyses reveal that bacteria promote plant defense during infection of soybean cyst nematode in soybean

BMC Plant Biol
PMCID: 5948838
PMID: 29751738
DOI: 10.1186/s12870-018-1302-9

[…] 2 × SYBR Master Premix ExTaq (Takara), 1 μl cDNA template (1:5 dilution), and 1 ul of each forward and reverse primer for the selected gene of interest. All primers were designed based on cDNAs using Primer Premier 5.0 (Additional file : Table S1). Three biological and three technical relipcates per sample were analysed using Bio-Rad Real-Time System (BioRad, Hercules, CA, USA) based on the 2-ΔΔCT […]


Genome wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs during Cryptosporidium baileyi infection

BMC Genomics
PMCID: 5946474
PMID: 29747577
DOI: 10.1186/s12864-018-4754-2

[…] r 10 s and annealing at 60 °C or 55 °C (Additional file ) for 30 s. All amplifications were followed by dissociation curve analysis of the amplified products. Specific primers were designed using the Primer Premier (v5.0), and specificities were confirmed with BLAST. The gene expression levels were normalized with the reference gene GAPDH by using 2−ΔΔCt value methods. The data were presented as t […]


Systematic analysis of DEMETER like DNA glycosylase genes shows lineage specific Smi miR7972 involved in SmDML1 regulation in Salvia miltiorrhiza

Sci Rep
PMCID: 5940787
PMID: 29739980
DOI: 10.1038/s41598-018-25315-w

[…] ned using a NanoDrop 2000C spectrophotometer (Thermo Scientific, USA). Reverse transcription was conducted using PrimeScript™ RT reagent kit (TaKaRa, Japan). Gene specific primers were designed using Primer Premier 6 (PREMIER Biosoft Int, USA) based on SmDML coding sequences. SmUBQ10 was used as an internal control as described previously. The expression of Smi-miR7972a and Smi-miR7972b was analyz […]

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Primer Premier institution(s)
Premier Biosoft International, Palo Alto, CA, USA; Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India

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